Somatostatin modulates the function of Kupffer cells

Tzu Chieh Chao; Hsiao Hsiang Chao; Miin Fu Chen; Jen Der Lin

doi:10.1016/S0167-0115(97)00008-6

Somatostatin modulates the function of Kupffer cells

Tzu Chieh Chao^*, Hsiao Hsiang Chao, Miin Fu Chen, Jen Der Lin

^*Corresponding author for this work

Chang Gung Memorial Hospital

Research output: Contribution to journal › Journal Article › peer-review

14 Scopus citations

Abstract

Recent studies have shown that somatostatin modulates lymphocyte and peritoneal macrophage function, but the effects of somatostatin on hepatic macrophages (Kupffer cells) are not clearly defined. In the present study, hepatic macrophages obtained from male rats were treated in vitro with somatostatin or octreotide and their effects on the release of nitrite, tumor necrosis factor (TNF), and hydrogen peroxide (H₂O₂) determined. At concentrations of 10¹⁴ M to 10¹²M, or greater than 10⁺¹⁰ M, somatostatin suppressed nitrite release by Kupffer cells. At concentrations of less than 10^-9M or greater than 10^-9M, octreotide inhibited nitrite release by Kupffer cells. Kupffer cells treated with 10^-10 M to 10^-14 M or greater than 10^-8 M of somatostatin released significantly less amounts of TNF than did the untreated controls. TNF release by Kupffer cells treated with 10^-15 M to 10^-5 M of octreotide was significantly inhibited as compared to that of untreated controls. Kupffer cells treated with 10^-14 M to 10^-11 and 10^-9 to 10^-8 M of somatostatin released more H₂O₂ than did the untreated controls. The amount of H₂O₂ released by noncirrhotic Kupffer cells treated with 10^-6 M or 10^-5 M of somatostatin was less than that of controls. These findings demonstrate that somatostatin and octreotide modulate the release of nitric oxide, TNF, H₂O be Kupffer cells depending on the concentration of hormones used.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Regulatory Peptides
Volume	69
Issue number	3
DOIs	https://doi.org/10.1016/S0167-0115(97)00008-6
State	Published - 30 04 1997
Externally published	Yes

Keywords

Hydrogen peroxide
Nitric oxide
Tumor necrosis factor

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10.1016/S0167-0115(97)00008-6

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title = "Somatostatin modulates the function of Kupffer cells",

abstract = "Recent studies have shown that somatostatin modulates lymphocyte and peritoneal macrophage function, but the effects of somatostatin on hepatic macrophages (Kupffer cells) are not clearly defined. In the present study, hepatic macrophages obtained from male rats were treated in vitro with somatostatin or octreotide and their effects on the release of nitrite, tumor necrosis factor (TNF), and hydrogen peroxide (H2O2) determined. At concentrations of 1014 M to 1012M, or greater than 10+10 M, somatostatin suppressed nitrite release by Kupffer cells. At concentrations of less than 10-9M or greater than 10-9M, octreotide inhibited nitrite release by Kupffer cells. Kupffer cells treated with 10-10 M to 10-14 M or greater than 10-8 M of somatostatin released significantly less amounts of TNF than did the untreated controls. TNF release by Kupffer cells treated with 10-15 M to 10-5 M of octreotide was significantly inhibited as compared to that of untreated controls. Kupffer cells treated with 10-14 M to 10-11 and 10-9 to 10-8 M of somatostatin released more H2O2 than did the untreated controls. The amount of H2O2 released by noncirrhotic Kupffer cells treated with 10-6 M or 10-5 M of somatostatin was less than that of controls. These findings demonstrate that somatostatin and octreotide modulate the release of nitric oxide, TNF, H2O be Kupffer cells depending on the concentration of hormones used.",

keywords = "Hydrogen peroxide, Nitric oxide, Tumor necrosis factor",

author = "Chao, {Tzu Chieh} and Chao, {Hsiao Hsiang} and Chen, {Miin Fu} and Lin, {Jen Der}",

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AU - Chao, Tzu Chieh

AU - Chao, Hsiao Hsiang

AU - Chen, Miin Fu

AU - Lin, Jen Der

PY - 1997/4/30

Y1 - 1997/4/30

N2 - Recent studies have shown that somatostatin modulates lymphocyte and peritoneal macrophage function, but the effects of somatostatin on hepatic macrophages (Kupffer cells) are not clearly defined. In the present study, hepatic macrophages obtained from male rats were treated in vitro with somatostatin or octreotide and their effects on the release of nitrite, tumor necrosis factor (TNF), and hydrogen peroxide (H2O2) determined. At concentrations of 1014 M to 1012M, or greater than 10+10 M, somatostatin suppressed nitrite release by Kupffer cells. At concentrations of less than 10-9M or greater than 10-9M, octreotide inhibited nitrite release by Kupffer cells. Kupffer cells treated with 10-10 M to 10-14 M or greater than 10-8 M of somatostatin released significantly less amounts of TNF than did the untreated controls. TNF release by Kupffer cells treated with 10-15 M to 10-5 M of octreotide was significantly inhibited as compared to that of untreated controls. Kupffer cells treated with 10-14 M to 10-11 and 10-9 to 10-8 M of somatostatin released more H2O2 than did the untreated controls. The amount of H2O2 released by noncirrhotic Kupffer cells treated with 10-6 M or 10-5 M of somatostatin was less than that of controls. These findings demonstrate that somatostatin and octreotide modulate the release of nitric oxide, TNF, H2O be Kupffer cells depending on the concentration of hormones used.

AB - Recent studies have shown that somatostatin modulates lymphocyte and peritoneal macrophage function, but the effects of somatostatin on hepatic macrophages (Kupffer cells) are not clearly defined. In the present study, hepatic macrophages obtained from male rats were treated in vitro with somatostatin or octreotide and their effects on the release of nitrite, tumor necrosis factor (TNF), and hydrogen peroxide (H2O2) determined. At concentrations of 1014 M to 1012M, or greater than 10+10 M, somatostatin suppressed nitrite release by Kupffer cells. At concentrations of less than 10-9M or greater than 10-9M, octreotide inhibited nitrite release by Kupffer cells. Kupffer cells treated with 10-10 M to 10-14 M or greater than 10-8 M of somatostatin released significantly less amounts of TNF than did the untreated controls. TNF release by Kupffer cells treated with 10-15 M to 10-5 M of octreotide was significantly inhibited as compared to that of untreated controls. Kupffer cells treated with 10-14 M to 10-11 and 10-9 to 10-8 M of somatostatin released more H2O2 than did the untreated controls. The amount of H2O2 released by noncirrhotic Kupffer cells treated with 10-6 M or 10-5 M of somatostatin was less than that of controls. These findings demonstrate that somatostatin and octreotide modulate the release of nitric oxide, TNF, H2O be Kupffer cells depending on the concentration of hormones used.

KW - Hydrogen peroxide

KW - Nitric oxide

KW - Tumor necrosis factor

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Somatostatin modulates the function of Kupffer cells

Abstract

Keywords

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