Spherical aberration cancellation in a polarized photon-pairs confocal laser scanning microscope

Chi Hui Chang, Chien Chou, Hsiu Fong Chang, Hon Fai Yau, Huan Jang Huang, Wen Chuan Kuo

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Refractive-index mismatch in conventional confocal microscopy produces severe degradation on axial resolution of sectioning image because the spherical aberration is generated in specimen. In this study, we propose a polarized photon-pairs confocal laser scanning microscope (PCLSM) in which a two-frequency linear polarized photon-pairs (LPPPs) laser beam is produced. The common-path propagation of LPPPs integrated with optical heterodyne technique not only can reduce the spherical aberration but also decreases scattering effect in specimen at same time. Therefore, the better axial and lateral resolutions of the sectioning image are produced simultaneously. In the experiment, a verification and comparison between PCLSM and conventional confocal laser scanning microscope (CLSM) on the ability of cancellation of spherical aberration induced by cover glass are demonstrated experimentally. Finally, the ability of PCLSM which can decrease the spherical aberration based on the common-path propagation of LPPPs associated with polarization gating, spatial coherence gating and spatial filtering gating is discussed.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2007
PublisherOptical Society of America
ISBN (Print)9780819467713
StatePublished - 2007
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2007 - Munich, Germany
Duration: 17 06 200717 06 2007

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2007
Country/TerritoryGermany
CityMunich
Period17/06/0717/06/07

Keywords

  • Confocal microscopy
  • Linearly polarized photon-pairs (LPPPs)
  • Optical heterodyne
  • Refractive-index mismatch
  • Spherical aberration cancellation

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