St. John's wort significantly increased the systemic exposure and toxicity of methotrexate in rats

Shih Ying Yang, Shin Hun Juang, Shang Yuan Tsai, Pei Dawn Lee Chao, Yu Chi Hou*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

16 Scopus citations

Abstract

St. John's wort (SJW, Hypericum perforatum) is one of the popular nutraceuticals for treating depression. Methotrexate (MTX) is an immunosuppressant with narrow therapeutic window. This study investigated the effect of SJW on MTX pharmacokinetics in rats.Rats were orally given MTX alone and coadministered with 300 and 150. mg/kg of SJW, and 25. mg/kg of diclofenac, respectively. Blood was withdrawn at specific time points and serum MTX concentrations were assayed by a specific monoclonal fluorescence polarization immunoassay method.The results showed that 300mg/kg of SJW significantly increased the AUC 0-t and C max of MTX by 163% and 60%, respectively, and 150mg/kg of SJW significantly increased the AUC 0-t of MTX by 55%. In addition, diclofenac enhanced the C max of MTX by 110%. The mortality of rats treated with SJW was higher than that of controls.In conclusion, coadministration of SJW significantly increased the systemic exposure and toxicity of MTX. The combined use of MTX with SJW would need to be with caution.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalToxicology and Applied Pharmacology
Volume263
Issue number1
DOIs
StatePublished - 15 08 2012
Externally publishedYes

Keywords

  • Hypericum perforatum
  • Methotrexate
  • Pharmacokinetics
  • St. John's wort

Fingerprint

Dive into the research topics of 'St. John's wort significantly increased the systemic exposure and toxicity of methotrexate in rats'. Together they form a unique fingerprint.

Cite this