TY - JOUR
T1 - Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis
AU - Heinsbroek, Sigrid E.M.
AU - Taylor, Philip R.
AU - Martinez, Fernando O.
AU - Martinez-Pomares, Luisa
AU - Brown, Gordon D.
AU - Gordon, Siamon
PY - 2008/11
Y1 - 2008/11
N2 - Candida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be involved in recognition and phagocytosis of this fungus, the relative role of these receptors has not been formally examined. In this paper, we have investigated the contribution of the mannose receptor, Dectin-1, and complement receptor 3; and we have demonstrated that Dectin-1 is the main non-opsonic receptor involved in fungal uptake. However, both Dectin-1 and complement receptor 3 were found to accumulate at the site of uptake, while mannose receptor accumulated on C. albicans phagosomes at later stages. These results suggest a potential role for MR in phagosome sampling; and, accordingly, MR deficiency led to a reduction in TNF-a and MCP-1 production in response to C. albicans uptake. Our data suggest that pattern recognition receptors sample the fungal phagosome in a sequential fashion.
AB - Candida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be involved in recognition and phagocytosis of this fungus, the relative role of these receptors has not been formally examined. In this paper, we have investigated the contribution of the mannose receptor, Dectin-1, and complement receptor 3; and we have demonstrated that Dectin-1 is the main non-opsonic receptor involved in fungal uptake. However, both Dectin-1 and complement receptor 3 were found to accumulate at the site of uptake, while mannose receptor accumulated on C. albicans phagosomes at later stages. These results suggest a potential role for MR in phagosome sampling; and, accordingly, MR deficiency led to a reduction in TNF-a and MCP-1 production in response to C. albicans uptake. Our data suggest that pattern recognition receptors sample the fungal phagosome in a sequential fashion.
UR - https://www.scopus.com/pages/publications/57149097072
U2 - 10.1371/journal.ppat.1000218
DO - 10.1371/journal.ppat.1000218
M3 - 文章
C2 - 19043561
AN - SCOPUS:57149097072
SN - 1553-7366
VL - 4
JO - PLoS Pathogens
JF - PLoS Pathogens
IS - 11
M1 - e1000218
ER -