STAT3 regulates the proliferation and differentiation of rabbit limbal epithelial cells via a ΔNp63-dependent mechanism

Yi Jen Hsueh; Hung Chi Chen; Wing Keung Chu; Chien Chia Cheng; Pei Ching Kuo; Liang Yu Lin; Hui Kang Ma; Jan Kan Chen

doi:10.1167/iovs.10-6103

STAT3 regulates the proliferation and differentiation of rabbit limbal epithelial cells via a ΔNp63-dependent mechanism

Yi Jen Hsueh, Hung Chi Chen, Wing Keung Chu, Chien Chia Cheng, Pei Ching Kuo, Liang Yu Lin, Hui Kang Ma, Jan Kan Chen^*

^*Corresponding author for this work

Department of Physiology

Research output: Contribution to journal › Journal Article › peer-review

22 Scopus citations

Abstract

Purpose. To explore the roles of STAT3 in the regulation of ΔNp63-dependent proliferation and differentiation of rabbit limbal keratinocytes. Methods. siRNAs were designed to specifically suppress the expression of STAT3 and ΔNp63, and their effects on limbal epithelial cell proliferation and differentiation were examined. Ectopically expressed ΔNp63 was used to compensate for the decreased endogenous ΔNp63. Immunoblot was used to examine the expressions of STAT3, ΔNp63, K3, integrin β1, and involucrin. Results. Limbal tissue expresses higher level of phosphorylated and nuclear translocated STAT3 compared with that of the cornea. Knockdown of STAT3 expression reduces the expression of ΔNp63, inhibits the expansion of limbal epithelial outgrowth, suppresses the expression of integrin β1, and promotes the expression of involucrin. Conclusions. STAT3 enhances the proliferation of limbal keratinocytes through a ΔNp63-dependent mechanism. Suppression of this pathway inhibits cell proliferation with a concomitant increase of cell differentiation.

Original language	English
Pages (from-to)	4685-4693
Number of pages	9
Journal	Investigative Ophthalmology and Visual Science
Volume	52
Issue number	7
DOIs	https://doi.org/10.1167/iovs.10-6103
State	Published - 06 2011

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@article{680a6736e5294170b5e7942265177f0b,

title = "STAT3 regulates the proliferation and differentiation of rabbit limbal epithelial cells via a ΔNp63-dependent mechanism",

abstract = "Purpose. To explore the roles of STAT3 in the regulation of ΔNp63-dependent proliferation and differentiation of rabbit limbal keratinocytes. Methods. siRNAs were designed to specifically suppress the expression of STAT3 and ΔNp63, and their effects on limbal epithelial cell proliferation and differentiation were examined. Ectopically expressed ΔNp63 was used to compensate for the decreased endogenous ΔNp63. Immunoblot was used to examine the expressions of STAT3, ΔNp63, K3, integrin β1, and involucrin. Results. Limbal tissue expresses higher level of phosphorylated and nuclear translocated STAT3 compared with that of the cornea. Knockdown of STAT3 expression reduces the expression of ΔNp63, inhibits the expansion of limbal epithelial outgrowth, suppresses the expression of integrin β1, and promotes the expression of involucrin. Conclusions. STAT3 enhances the proliferation of limbal keratinocytes through a ΔNp63-dependent mechanism. Suppression of this pathway inhibits cell proliferation with a concomitant increase of cell differentiation.",

author = "Hsueh, {Yi Jen} and Chen, {Hung Chi} and Chu, {Wing Keung} and Cheng, {Chien Chia} and Kuo, {Pei Ching} and Lin, {Liang Yu} and Ma, {Hui Kang} and Chen, {Jan Kan}",

year = "2011",

month = jun,

doi = "10.1167/iovs.10-6103",

language = "英语",

volume = "52",

pages = "4685--4693",

journal = "Investigative Ophthalmology and Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology Inc.",

number = "7",

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TY - JOUR

T1 - STAT3 regulates the proliferation and differentiation of rabbit limbal epithelial cells via a ΔNp63-dependent mechanism

AU - Hsueh, Yi Jen

AU - Chen, Hung Chi

AU - Chu, Wing Keung

AU - Cheng, Chien Chia

AU - Kuo, Pei Ching

AU - Lin, Liang Yu

AU - Ma, Hui Kang

AU - Chen, Jan Kan

PY - 2011/6

Y1 - 2011/6

N2 - Purpose. To explore the roles of STAT3 in the regulation of ΔNp63-dependent proliferation and differentiation of rabbit limbal keratinocytes. Methods. siRNAs were designed to specifically suppress the expression of STAT3 and ΔNp63, and their effects on limbal epithelial cell proliferation and differentiation were examined. Ectopically expressed ΔNp63 was used to compensate for the decreased endogenous ΔNp63. Immunoblot was used to examine the expressions of STAT3, ΔNp63, K3, integrin β1, and involucrin. Results. Limbal tissue expresses higher level of phosphorylated and nuclear translocated STAT3 compared with that of the cornea. Knockdown of STAT3 expression reduces the expression of ΔNp63, inhibits the expansion of limbal epithelial outgrowth, suppresses the expression of integrin β1, and promotes the expression of involucrin. Conclusions. STAT3 enhances the proliferation of limbal keratinocytes through a ΔNp63-dependent mechanism. Suppression of this pathway inhibits cell proliferation with a concomitant increase of cell differentiation.

AB - Purpose. To explore the roles of STAT3 in the regulation of ΔNp63-dependent proliferation and differentiation of rabbit limbal keratinocytes. Methods. siRNAs were designed to specifically suppress the expression of STAT3 and ΔNp63, and their effects on limbal epithelial cell proliferation and differentiation were examined. Ectopically expressed ΔNp63 was used to compensate for the decreased endogenous ΔNp63. Immunoblot was used to examine the expressions of STAT3, ΔNp63, K3, integrin β1, and involucrin. Results. Limbal tissue expresses higher level of phosphorylated and nuclear translocated STAT3 compared with that of the cornea. Knockdown of STAT3 expression reduces the expression of ΔNp63, inhibits the expansion of limbal epithelial outgrowth, suppresses the expression of integrin β1, and promotes the expression of involucrin. Conclusions. STAT3 enhances the proliferation of limbal keratinocytes through a ΔNp63-dependent mechanism. Suppression of this pathway inhibits cell proliferation with a concomitant increase of cell differentiation.

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U2 - 10.1167/iovs.10-6103

DO - 10.1167/iovs.10-6103

M3 - 文章

C2 - 21447682

AN - SCOPUS:80052361051

SN - 0146-0404

VL - 52

SP - 4685

EP - 4693

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

IS - 7

ER -

STAT3 regulates the proliferation and differentiation of rabbit limbal epithelial cells via a ΔNp63-dependent mechanism

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