TY - JOUR
T1 - Steroid Sex Hormones and Macrophage Function
T2 - Modulation of Reactive Oxygen Intermediates and Nitrite Release
AU - Chao, Tzu‐Chieh ‐C
AU - Alten, Pierson J.Van
AU - Walter, Robert J.
PY - 1994/8
Y1 - 1994/8
N2 - PROBLEM: In general, females have a more active immune response than do males. The effects of female sex hormones on lymphocytes have been studied extensively but their effects on macrophages are poorly understood. METHOD: In this study, peritoneal macrophages (M0) obtained from male rats were treated in vitro with estradiol (E2), progesterone (P), testosterone (TS), or hydrocortisone (HC) and their effects on superoxide, hydrogen peroxide, and nitrite release determined. RESULTS: At concentrations between 10−10 and 10−9 M, female and male sex hormones had no significant effect on superoxide release but, at concentrations above or below that range, these hormones stimulated the release of these reactive oxygen intermediates (ROI). In contrast, M0 treated with HC generally exhibited either unaltered or reduced ROI release. CONCLUSIONS: These findings suggest that female sex hormones regulate ROI release by Mø in a manner not entirely shared by other steroid hormones. At most concentrations used, E2, P, TS, and HC significantly inhibited nitrite release by Mø. However, with 10−10 M of E2 or 10−9M of P, nitrite release by Mø was not affected. In the presence of anti‐TNF antibody, the amounts of superoxide and hydrogen peroxide release were moderately reduced but nitrite release was dramatically inhibited. The sensitivity of Mø to variations in the concentrations of female sex hormones may contribute to gender‐related differences in the immune response. 1994 Munksgaard
AB - PROBLEM: In general, females have a more active immune response than do males. The effects of female sex hormones on lymphocytes have been studied extensively but their effects on macrophages are poorly understood. METHOD: In this study, peritoneal macrophages (M0) obtained from male rats were treated in vitro with estradiol (E2), progesterone (P), testosterone (TS), or hydrocortisone (HC) and their effects on superoxide, hydrogen peroxide, and nitrite release determined. RESULTS: At concentrations between 10−10 and 10−9 M, female and male sex hormones had no significant effect on superoxide release but, at concentrations above or below that range, these hormones stimulated the release of these reactive oxygen intermediates (ROI). In contrast, M0 treated with HC generally exhibited either unaltered or reduced ROI release. CONCLUSIONS: These findings suggest that female sex hormones regulate ROI release by Mø in a manner not entirely shared by other steroid hormones. At most concentrations used, E2, P, TS, and HC significantly inhibited nitrite release by Mø. However, with 10−10 M of E2 or 10−9M of P, nitrite release by Mø was not affected. In the presence of anti‐TNF antibody, the amounts of superoxide and hydrogen peroxide release were moderately reduced but nitrite release was dramatically inhibited. The sensitivity of Mø to variations in the concentrations of female sex hormones may contribute to gender‐related differences in the immune response. 1994 Munksgaard
KW - Estrogen
KW - macrophages
KW - nitrite
KW - progesterone
KW - respiratory burst
KW - steroid hormone
KW - superoxide
UR - http://www.scopus.com/inward/record.url?scp=0028363990&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0897.1994.tb00877.x
DO - 10.1111/j.1600-0897.1994.tb00877.x
M3 - 文章
C2 - 7945814
AN - SCOPUS:0028363990
SN - 1046-7408
VL - 32
SP - 43
EP - 52
JO - American Journal of Reproductive Immunology
JF - American Journal of Reproductive Immunology
IS - 1
ER -