Stimulation of the cytosolic receptor for peptidoglycan, Nod1, by infection with Chlamydia trachomatis or Chlamydia muridarum

Lynn Welter-Stahl, David M. Ojcius*, Jérôme Viala, Stéphane Girardin, Wei Liu, Christiane Delarbre, Dana Philpott, Kathleen A. Kelly, Toni Darville

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

118 Scopus citations

Abstract

Infection of epithelial cells by the intracellular pathogen, Chlamydia trachomatis, leads to activation of NF-κB and secretion of pro-inflammatory cytokines. We find that overexpression of a dominant-negative Nod1 or depletion of Nod1 by RNA interference inhibits partially the activation of NF-κB during chlamydial infection in vitro, suggesting that Nod1 can detect the presence of Chlamydia. In parallel, there is a larger increase in the expression of pro-inflammatory genes following Chlamydia infection when primary fibroblasts are isolated from wild-type mice than from Nod1-deficient mice. The Chlamydia genome encodes all the putative enzymes required for proteoglycan synthesis, but proteoglycan from Chlamydia has never been detected biochemically. Since Nod1 is a ubiquitous cytosolic receptor for peptidoglycan from Gram-negative bacteria, our results suggest that C. trachomatis and C. muridarum do in fact produce at least the rudimentary proteoglycan motif recognized by Nod1. Nonetheless, Nod1 deficiency has no effect on the efficiency of infection, the intensity of cytokine secretion, or pathology in vaginally infected mice, compared with wild-type controls. Similarly, Rip2, a downstream mediator of Nod1, Toll-like receptor (TLR)-2, and TLR4, increases only slightly the intensity of chlamydial infection in vivo and has a very mild effect on the immune response and pathology. Thus, Chlamydia may not produce sufficient peptidoglycan to stimulate Nod1-dependent pathways efficiently in infected animals, or other receptors of the innate immune system may compensate for the absence of Nod1 during Chlamydia infection in vivo.

Original languageEnglish
Pages (from-to)1047-1057
Number of pages11
JournalCellular Microbiology
Volume8
Issue number6
DOIs
StatePublished - 06 2006
Externally publishedYes

Fingerprint

Dive into the research topics of 'Stimulation of the cytosolic receptor for peptidoglycan, Nod1, by infection with Chlamydia trachomatis or Chlamydia muridarum'. Together they form a unique fingerprint.

Cite this