Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants

E. K.W. Hui; P. C. Wang; S. J. Lo

doi:10.1007/s000180050262

Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants

E. K.W. Hui, P. C. Wang, S. J. Lo^*

^*Corresponding author for this work

National Yang Ming Chiao Tung University

Research output: Contribution to journal › Review article › peer-review

66 Scopus citations

Abstract

Many virus and transposon DNAs call integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.

Original language	English
Pages (from-to)	1403-1411
Number of pages	9
Journal	Cellular and Molecular Life Sciences
Volume	54
Issue number	12
DOIs	https://doi.org/10.1007/s000180050262
State	Published - 1998
Externally published	Yes

Keywords

Boomerang DNA amplification (BDA)
Capture PCR (CPCR)
Inverse PCR (IPCR)
Novel alu-PCR
Panhandle PCR
Polymerase chain reaction (PCR)
Restriction-site PCR (RS-PCR)
Splinkerette-PCR
Targeted gene-walking PCR
Vectorette-PCR

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title = "Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants",

abstract = "Many virus and transposon DNAs call integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.",

keywords = "Boomerang DNA amplification (BDA), Capture PCR (CPCR), Inverse PCR (IPCR), Novel alu-PCR, Panhandle PCR, Polymerase chain reaction (PCR), Restriction-site PCR (RS-PCR), Splinkerette-PCR, Targeted gene-walking PCR, Vectorette-PCR",

author = "Hui, {E. K.W.} and Wang, {P. C.} and Lo, {S. J.}",

year = "1998",

doi = "10.1007/s000180050262",

language = "英语",

volume = "54",

pages = "1403--1411",

journal = "Cellular and Molecular Life Sciences",

issn = "1420-682X",

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TY - JOUR

T1 - Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants

AU - Hui, E. K.W.

AU - Wang, P. C.

AU - Lo, S. J.

PY - 1998

Y1 - 1998

N2 - Many virus and transposon DNAs call integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.

AB - Many virus and transposon DNAs call integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.

KW - Boomerang DNA amplification (BDA)

KW - Capture PCR (CPCR)

KW - Inverse PCR (IPCR)

KW - Novel alu-PCR

KW - Panhandle PCR

KW - Polymerase chain reaction (PCR)

KW - Restriction-site PCR (RS-PCR)

KW - Splinkerette-PCR

KW - Targeted gene-walking PCR

KW - Vectorette-PCR

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U2 - 10.1007/s000180050262

DO - 10.1007/s000180050262

M3 - 文献综述

C2 - 9893713

AN - SCOPUS:0032431243

SN - 1420-682X

VL - 54

SP - 1403

EP - 1411

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

IS - 12

ER -

Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants

Abstract

Keywords

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