Abstract
Many virus and transposon DNAs call integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1403-1411 |
| Number of pages | 9 |
| Journal | Cellular and Molecular Life Sciences |
| Volume | 54 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
Keywords
- Boomerang DNA amplification (BDA)
- Capture PCR (CPCR)
- Inverse PCR (IPCR)
- Novel alu-PCR
- Panhandle PCR
- Polymerase chain reaction (PCR)
- Restriction-site PCR (RS-PCR)
- Splinkerette-PCR
- Targeted gene-walking PCR
- Vectorette-PCR