TY - JOUR
T1 - Streptococcus salivarius urease expression
T2 - involvement of the phosphoenolpyruvate:sugar phosphotransferase system
AU - Chen, Yi Ywan M.
AU - Hall, Thomas H.
AU - Burne, Robert A.
PY - 1998/8/1
Y1 - 1998/8/1
N2 - Urease expression in Streptococcus salivarius 57.I is induced by acidic pH, and further enhanced at high growth rate and with excess carbohydrate. Notably, the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) activity is repressed in oral streptococci under the same conditions. To test the hypothesis that the PTS may be involved in urease regulation, spontaneous mutants (PTS-1 and PTS-4) that were resistant to 2-deoxyglucose were isolated. When compared to wild-type, PTS-1 was devoid of enzyme IIA(Man)(H) (EIIA(Man)(H)) and synthesized low amounts of EIIA(Man)(L), and PTS-4 was devoid of EIIA(Man)(L), but produced wild-type levels of EIIA(Man)(H). Urease expression was examined in continuous chemostat cultures at steady state. Induction by acidic pH was still observed in both mutants, but at lower levels compared to wild-type, under carbohydrate limiting conditions. Conversely, the lower level of expression in PTS-4 could be overcome in excess carbohydrate. The data indicated evidence of a molecular link between the PTS, sugar metabolism, and regulation of urease expression. Copyright (C) 1998 Federation of European Microbiological Societies.
AB - Urease expression in Streptococcus salivarius 57.I is induced by acidic pH, and further enhanced at high growth rate and with excess carbohydrate. Notably, the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS) activity is repressed in oral streptococci under the same conditions. To test the hypothesis that the PTS may be involved in urease regulation, spontaneous mutants (PTS-1 and PTS-4) that were resistant to 2-deoxyglucose were isolated. When compared to wild-type, PTS-1 was devoid of enzyme IIA(Man)(H) (EIIA(Man)(H)) and synthesized low amounts of EIIA(Man)(L), and PTS-4 was devoid of EIIA(Man)(L), but produced wild-type levels of EIIA(Man)(H). Urease expression was examined in continuous chemostat cultures at steady state. Induction by acidic pH was still observed in both mutants, but at lower levels compared to wild-type, under carbohydrate limiting conditions. Conversely, the lower level of expression in PTS-4 could be overcome in excess carbohydrate. The data indicated evidence of a molecular link between the PTS, sugar metabolism, and regulation of urease expression. Copyright (C) 1998 Federation of European Microbiological Societies.
KW - Gene regulation
KW - Phosphotransferase system
KW - Streptococcus salivarius
KW - Urease
UR - http://www.scopus.com/inward/record.url?scp=0032145506&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(98)00261-4
DO - 10.1016/S0378-1097(98)00261-4
M3 - 文章
C2 - 9711847
AN - SCOPUS:0032145506
SN - 0378-1097
VL - 165
SP - 117
EP - 122
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -