Structure-function relationship of trichosanthin

Yi Bao Ke; Jan Kan Chen; Hui Ling Nie; Xian Hui He; Xin Yong Ke; Ya Hui Wang

doi:10.1016/S0024-3205(96)00676-5

Structure-function relationship of trichosanthin

Yi Bao Ke, Jan Kan Chen^*, Hui Ling Nie, Xian Hui He, Xin Yong Ke, Ya Hui Wang

^*Corresponding author for this work

Department of Physiology

Research output: Contribution to journal › Journal Article › peer-review

11 Scopus citations

Abstract

Trichosanthin (TCS), a 27 kDa ribosome inactivating protein extracted from the root tuber of Trichosanthes plant, was subjected to limited chymotrypsin digestion and three peptide fragments designated TCS-F1 72-247, TCS-F2 100-247, and TCS-F3 133-247 were generated. The RNA-N glycosidase and cytotoxic activities of the TCS fragments were compared with that of intact TCS. TCS-F1 and TCS-F2 were biologically active, while TCS-F3 was completely inactive. Dose-dependent studies showed that TCS-F1 and TCS-F2 were less potent in their trophoblast cytotoxicity than intact TCS, however, full biological effect could still be obtained with a higher dosage. Based on the known three-dimensional structure of TCS, we postulate that the putative active site of TCS is located at amino acid residues 110 to 174.

Original language	English
Pages (from-to)	465-472
Number of pages	8
Journal	Life Sciences
Volume	60
Issue number	7
DOIs	https://doi.org/10.1016/S0024-3205(96)00676-5
State	Published - 10 01 1997

Keywords

cytotoxicity
ribosome inactivation
trichosanthin
trophoblasts

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10.1016/S0024-3205(96)00676-5

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@article{f72208b0c89141a487177570676e95c2,

title = "Structure-function relationship of trichosanthin",

abstract = "Trichosanthin (TCS), a 27 kDa ribosome inactivating protein extracted from the root tuber of Trichosanthes plant, was subjected to limited chymotrypsin digestion and three peptide fragments designated TCS-F1 72-247, TCS-F2 100-247, and TCS-F3 133-247 were generated. The RNA-N glycosidase and cytotoxic activities of the TCS fragments were compared with that of intact TCS. TCS-F1 and TCS-F2 were biologically active, while TCS-F3 was completely inactive. Dose-dependent studies showed that TCS-F1 and TCS-F2 were less potent in their trophoblast cytotoxicity than intact TCS, however, full biological effect could still be obtained with a higher dosage. Based on the known three-dimensional structure of TCS, we postulate that the putative active site of TCS is located at amino acid residues 110 to 174.",

keywords = "cytotoxicity, ribosome inactivation, trichosanthin, trophoblasts",

author = "Ke, {Yi Bao} and Chen, {Jan Kan} and Nie, {Hui Ling} and He, {Xian Hui} and Ke, {Xin Yong} and Wang, {Ya Hui}",

year = "1997",

month = jan,

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doi = "10.1016/S0024-3205(96)00676-5",

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T1 - Structure-function relationship of trichosanthin

AU - Ke, Yi Bao

AU - Chen, Jan Kan

AU - Nie, Hui Ling

AU - He, Xian Hui

AU - Ke, Xin Yong

AU - Wang, Ya Hui

PY - 1997/1/10

Y1 - 1997/1/10

N2 - Trichosanthin (TCS), a 27 kDa ribosome inactivating protein extracted from the root tuber of Trichosanthes plant, was subjected to limited chymotrypsin digestion and three peptide fragments designated TCS-F1 72-247, TCS-F2 100-247, and TCS-F3 133-247 were generated. The RNA-N glycosidase and cytotoxic activities of the TCS fragments were compared with that of intact TCS. TCS-F1 and TCS-F2 were biologically active, while TCS-F3 was completely inactive. Dose-dependent studies showed that TCS-F1 and TCS-F2 were less potent in their trophoblast cytotoxicity than intact TCS, however, full biological effect could still be obtained with a higher dosage. Based on the known three-dimensional structure of TCS, we postulate that the putative active site of TCS is located at amino acid residues 110 to 174.

AB - Trichosanthin (TCS), a 27 kDa ribosome inactivating protein extracted from the root tuber of Trichosanthes plant, was subjected to limited chymotrypsin digestion and three peptide fragments designated TCS-F1 72-247, TCS-F2 100-247, and TCS-F3 133-247 were generated. The RNA-N glycosidase and cytotoxic activities of the TCS fragments were compared with that of intact TCS. TCS-F1 and TCS-F2 were biologically active, while TCS-F3 was completely inactive. Dose-dependent studies showed that TCS-F1 and TCS-F2 were less potent in their trophoblast cytotoxicity than intact TCS, however, full biological effect could still be obtained with a higher dosage. Based on the known three-dimensional structure of TCS, we postulate that the putative active site of TCS is located at amino acid residues 110 to 174.

KW - cytotoxicity

KW - ribosome inactivation

KW - trichosanthin

KW - trophoblasts

UR - http://www.scopus.com/inward/record.url?scp=0031561811&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(96)00676-5

DO - 10.1016/S0024-3205(96)00676-5

M3 - 文章

C2 - 9042374

AN - SCOPUS:0031561811

SN - 0024-3205

VL - 60

SP - 465

EP - 472

JO - Life Sciences

JF - Life Sciences

IS - 7

ER -

Structure-function relationship of trichosanthin

Abstract

Keywords

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