Study of on-line monitoring of lactate based on optical fibre sensor and in-channel mixing mechanism

Min Hsien Wu; Junbo Wang; Taha Taha; Zhanfeng Cui; Jill P.G. Urban; Zheng Cui

doi:10.1007/s10544-006-9018-2

Study of on-line monitoring of lactate based on optical fibre sensor and in-channel mixing mechanism

Min Hsien Wu
, Junbo Wang
, Taha Taha
, Zhanfeng Cui^*
, Jill P.G. Urban
, Zheng Cui^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

25 Scopus citations

Abstract

A PDMS-based microfluidic device with integrated optical fibres was developed for online monitoring of lactate. The detection is based on the optical adsorption of the colour solution formed by in-channel mixing of sample and reagent solutions. Computational Fluid Dynamics (CFD) simulation and kinetic study of the colour development were conducted to determine the minimum channel length and optimum residential time for homogenous mixing and maximum optical adsorption respectively. The system was proved to be able to detect lactate with good linearity (R² = 0.98), response time of about 130 s and estimated limitation of detection (LOD) of 0.52 mM (about 47 mg/L), which is sufficient for a general online lactate detection. Due to the miniaturization of sensing system and micro-scale mixing, higher detection sensitivity (0.15 V/mM) can be realized than the mixing in lab-scale equipments (0.08 V/mM).

Original language	English
Pages (from-to)	167-174
Number of pages	8
Journal	Biomedical Microdevices
Volume	9
Issue number	2
DOIs	https://doi.org/10.1007/s10544-006-9018-2
State	Published - 04 2007
Externally published	Yes

Keywords

Lactate
Microfluidic devices
On-line monitoring
Optical fibre
PDMS
Sensor

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10.1007/s10544-006-9018-2

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@article{b28ac5a8ddca46ce818497419e99200f,

title = "Study of on-line monitoring of lactate based on optical fibre sensor and in-channel mixing mechanism",

abstract = "A PDMS-based microfluidic device with integrated optical fibres was developed for online monitoring of lactate. The detection is based on the optical adsorption of the colour solution formed by in-channel mixing of sample and reagent solutions. Computational Fluid Dynamics (CFD) simulation and kinetic study of the colour development were conducted to determine the minimum channel length and optimum residential time for homogenous mixing and maximum optical adsorption respectively. The system was proved to be able to detect lactate with good linearity (R2 = 0.98), response time of about 130 s and estimated limitation of detection (LOD) of 0.52 mM (about 47 mg/L), which is sufficient for a general online lactate detection. Due to the miniaturization of sensing system and micro-scale mixing, higher detection sensitivity (0.15 V/mM) can be realized than the mixing in lab-scale equipments (0.08 V/mM).",

keywords = "Lactate, Microfluidic devices, On-line monitoring, Optical fibre, PDMS, Sensor",

author = "Wu, \{Min Hsien\} and Junbo Wang and Taha Taha and Zhanfeng Cui and Urban, \{Jill P.G.\} and Zheng Cui",

year = "2007",

month = apr,

doi = "10.1007/s10544-006-9018-2",

language = "英语",

volume = "9",

pages = "167--174",

journal = "Biomedical Microdevices",

issn = "1387-2176",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Study of on-line monitoring of lactate based on optical fibre sensor and in-channel mixing mechanism

AU - Wu, Min Hsien

AU - Wang, Junbo

AU - Taha, Taha

AU - Cui, Zhanfeng

AU - Urban, Jill P.G.

AU - Cui, Zheng

PY - 2007/4

Y1 - 2007/4

N2 - A PDMS-based microfluidic device with integrated optical fibres was developed for online monitoring of lactate. The detection is based on the optical adsorption of the colour solution formed by in-channel mixing of sample and reagent solutions. Computational Fluid Dynamics (CFD) simulation and kinetic study of the colour development were conducted to determine the minimum channel length and optimum residential time for homogenous mixing and maximum optical adsorption respectively. The system was proved to be able to detect lactate with good linearity (R2 = 0.98), response time of about 130 s and estimated limitation of detection (LOD) of 0.52 mM (about 47 mg/L), which is sufficient for a general online lactate detection. Due to the miniaturization of sensing system and micro-scale mixing, higher detection sensitivity (0.15 V/mM) can be realized than the mixing in lab-scale equipments (0.08 V/mM).

AB - A PDMS-based microfluidic device with integrated optical fibres was developed for online monitoring of lactate. The detection is based on the optical adsorption of the colour solution formed by in-channel mixing of sample and reagent solutions. Computational Fluid Dynamics (CFD) simulation and kinetic study of the colour development were conducted to determine the minimum channel length and optimum residential time for homogenous mixing and maximum optical adsorption respectively. The system was proved to be able to detect lactate with good linearity (R2 = 0.98), response time of about 130 s and estimated limitation of detection (LOD) of 0.52 mM (about 47 mg/L), which is sufficient for a general online lactate detection. Due to the miniaturization of sensing system and micro-scale mixing, higher detection sensitivity (0.15 V/mM) can be realized than the mixing in lab-scale equipments (0.08 V/mM).

KW - Lactate

KW - Microfluidic devices

KW - On-line monitoring

KW - Optical fibre

KW - PDMS

KW - Sensor

UR - https://www.scopus.com/pages/publications/34147136188

U2 - 10.1007/s10544-006-9018-2

DO - 10.1007/s10544-006-9018-2

M3 - 文章

C2 - 17160706

AN - SCOPUS:34147136188

SN - 1387-2176

VL - 9

SP - 167

EP - 174

JO - Biomedical Microdevices

JF - Biomedical Microdevices

IS - 2

ER -

Study of on-line monitoring of lactate based on optical fibre sensor and in-channel mixing mechanism

Abstract

Keywords

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