Suppressed TGF-β1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment

Yur Ren Kuo; Wen Shan Wu; Seag Feng Jeng; Feng Sheng Wang; Hui Chen Huang; Cha Zon Lin; Kuender D. Yang

doi:10.1002/lsm.20104

Suppressed TGF-β1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment

Yur Ren Kuo, Wen Shan Wu, Seag Feng Jeng, Feng Sheng Wang, Hui Chen Huang, Cha Zon Lin, Kuender D. Yang^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

102 Scopus citations

Abstract

Background and Objectives: Flashlamp pulsed-dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor-β1 (TGF-β1) - induction and up-regulation of matrix metalloproteinase (MMP) expression in keloid regression. Study Design/Materials and Methods: Keloid tissues obtained from 10 patients with intra-lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10-18 J/cm² (mean 14.0 J/cm²)]. Immunohistochemical (IHC) staining of TGF-β1 and MMP-1 and MMP-13 expressions in keloid tissue was performed. Western blot analysis of MMP-1 and MMP-13 expressions in extracellular matrix was evaluated. Results: IHC staining indicated that expression of TGF-β1 was significantly reduced in keloid tissues after PDL irradiation. MMP-13 but not MMP-1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP-13 but not MMP-1 significant increased in keloid tissues after PDL treatment. Conclusions: Regression of keloids regressed after PDL treatments are associated with down-regulation of TGF-β1 expression and up-regulation of MMP-13 activity.

Original language	English
Pages (from-to)	38-42
Number of pages	5
Journal	Lasers in Surgery and Medicine
Volume	36
Issue number	1
DOIs	https://doi.org/10.1002/lsm.20104
State	Published - 01 2005
Externally published	Yes

Keywords

Keloids
Matrix metalloproteinase
Pulsed-dye laser
TGF-β1

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title = "Suppressed TGF-β1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment",

abstract = "Background and Objectives: Flashlamp pulsed-dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor-β1 (TGF-β1) - induction and up-regulation of matrix metalloproteinase (MMP) expression in keloid regression. Study Design/Materials and Methods: Keloid tissues obtained from 10 patients with intra-lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10-18 J/cm2 (mean 14.0 J/cm2)]. Immunohistochemical (IHC) staining of TGF-β1 and MMP-1 and MMP-13 expressions in keloid tissue was performed. Western blot analysis of MMP-1 and MMP-13 expressions in extracellular matrix was evaluated. Results: IHC staining indicated that expression of TGF-β1 was significantly reduced in keloid tissues after PDL irradiation. MMP-13 but not MMP-1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP-13 but not MMP-1 significant increased in keloid tissues after PDL treatment. Conclusions: Regression of keloids regressed after PDL treatments are associated with down-regulation of TGF-β1 expression and up-regulation of MMP-13 activity.",

keywords = "Keloids, Matrix metalloproteinase, Pulsed-dye laser, TGF-β1",

author = "Kuo, {Yur Ren} and Wu, {Wen Shan} and Jeng, {Seag Feng} and Wang, {Feng Sheng} and Huang, {Hui Chen} and Lin, {Cha Zon} and Yang, {Kuender D.}",

year = "2005",

month = jan,

doi = "10.1002/lsm.20104",

language = "英语",

volume = "36",

pages = "38--42",

journal = "Lasers in Surgery and Medicine",

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publisher = "Wiley-Liss Inc.",

number = "1",

}

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T1 - Suppressed TGF-β1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment

AU - Kuo, Yur Ren

AU - Wu, Wen Shan

AU - Jeng, Seag Feng

AU - Wang, Feng Sheng

AU - Huang, Hui Chen

AU - Lin, Cha Zon

AU - Yang, Kuender D.

PY - 2005/1

Y1 - 2005/1

N2 - Background and Objectives: Flashlamp pulsed-dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor-β1 (TGF-β1) - induction and up-regulation of matrix metalloproteinase (MMP) expression in keloid regression. Study Design/Materials and Methods: Keloid tissues obtained from 10 patients with intra-lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10-18 J/cm2 (mean 14.0 J/cm2)]. Immunohistochemical (IHC) staining of TGF-β1 and MMP-1 and MMP-13 expressions in keloid tissue was performed. Western blot analysis of MMP-1 and MMP-13 expressions in extracellular matrix was evaluated. Results: IHC staining indicated that expression of TGF-β1 was significantly reduced in keloid tissues after PDL irradiation. MMP-13 but not MMP-1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP-13 but not MMP-1 significant increased in keloid tissues after PDL treatment. Conclusions: Regression of keloids regressed after PDL treatments are associated with down-regulation of TGF-β1 expression and up-regulation of MMP-13 activity.

AB - Background and Objectives: Flashlamp pulsed-dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor-β1 (TGF-β1) - induction and up-regulation of matrix metalloproteinase (MMP) expression in keloid regression. Study Design/Materials and Methods: Keloid tissues obtained from 10 patients with intra-lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10-18 J/cm2 (mean 14.0 J/cm2)]. Immunohistochemical (IHC) staining of TGF-β1 and MMP-1 and MMP-13 expressions in keloid tissue was performed. Western blot analysis of MMP-1 and MMP-13 expressions in extracellular matrix was evaluated. Results: IHC staining indicated that expression of TGF-β1 was significantly reduced in keloid tissues after PDL irradiation. MMP-13 but not MMP-1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP-13 but not MMP-1 significant increased in keloid tissues after PDL treatment. Conclusions: Regression of keloids regressed after PDL treatments are associated with down-regulation of TGF-β1 expression and up-regulation of MMP-13 activity.

KW - Keloids

KW - Matrix metalloproteinase

KW - Pulsed-dye laser

KW - TGF-β1

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Suppressed TGF-β1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment

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