Suppression of the tumorigenicity of human hepatoma hep3B cells by long-term retinoic acid treatment

Shih Lan Hsu, Hsing Mei Lin, Chen Kung Chou*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

15 Scopus citations

Abstract

We cultured human hepatoma Hep3B cells in the presence of RA (10-5 M) for 30 days; the expression of both α-fetoprotein and hepatitis B virus surface antigen were suppressed over 70% at the transcriptional level by RA treatment. The doubling time of RA treated Hep3B cells was slightly different from the control cells when they were cultured in 5% fetal calf serum/DMEM medium. However, cultured under serum-free conditions, the control Hep3B cells still grow, but the RA treated cells could not attach to the substratum of the culture plate and stopped growing. In vivo assay indicated that RA treatment completely suppressed the tumorigenicity of Hep3B cells in nude mice.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalCancer Letters
Volume99
Issue number1
DOIs
StatePublished - 13 01 1996
Externally publishedYes

Keywords

  • Hep3B cell
  • Hepatitis B virus surface antigen
  • Retinoic acid
  • α-fetoprotein

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