Surface plasmon resonance biochips for tuberculosis bacillus detection

Jhen Gang Huang, Chi Chan K. Hung, Hsin Chih Lai, Chih Kung Lee, Shi Ming Lin, Ping Feng, Chii Wann Lin*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations

Abstract

Tuberculosis is one of notifiable infectious diseases which may cause serious epidemic problems. Traditional diagnostic techniques include acid- fast stain, bacteria culture, metabolic monitoring have some disadvantages such as low sensitivity, time consumption and less specificity. In this study we proposed a new diagnostic technique based on the protein chips concept which detected by SPR phenomena. It was found that the resonance angle (∼51.86°) shifted slightly toward right with antibody concentration of 10X, 30X, 100X, and 300X dilution under optimal concentration of immobilized TB antigen W38 (MW 41.5 kDa, 50 μg/ml). While for the W06 (MW 14 kDa, 50 μg/ml), the resonance angle was around 50.13° and shifted with the same trend as W38 did.

Original languageEnglish
Pages (from-to)2553-2556
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume26 IV
StatePublished - 2004
Externally publishedYes
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: 01 09 200405 09 2004

Keywords

  • Protein chip
  • SPR
  • Tuberculosis bacillus

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