Abstract
Tuberculosis is one of notifiable infectious diseases which may cause serious epidemic problems. Traditional diagnostic techniques include acid- fast stain, bacteria culture, metabolic monitoring have some disadvantages such as low sensitivity, time consumption and less specificity. In this study we proposed a new diagnostic technique based on the protein chips concept which detected by SPR phenomena. It was found that the resonance angle (∼51.86°) shifted slightly toward right with antibody concentration of 10X, 30X, 100X, and 300X dilution under optimal concentration of immobilized TB antigen W38 (MW 41.5 kDa, 50 μg/ml). While for the W06 (MW 14 kDa, 50 μg/ml), the resonance angle was around 50.13° and shifted with the same trend as W38 did.
Original language | English |
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Pages (from-to) | 2553-2556 |
Number of pages | 4 |
Journal | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
Volume | 26 IV |
State | Published - 2004 |
Externally published | Yes |
Event | Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States Duration: 01 09 2004 → 05 09 2004 |
Keywords
- Protein chip
- SPR
- Tuberculosis bacillus