Surfactant effects on lipase-catalyzed hydrolysis of olive oil in AOT/ISOOCTANE reverse micelles

Shau Wei Tsai; Kung Ping Lee; Chen Li Chiang

doi:10.3109/10242429509015215

Surfactant effects on lipase-catalyzed hydrolysis of olive oil in AOT/ISOOCTANE reverse micelles

Shau Wei Tsai^*, Kung Ping Lee, Chen Li Chiang

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

8 Scopus citations

Abstract

The effects of surfactant concentration on the hydrolytic activity of Candida rugosa lipase in AOT/isooctane reverse micelles with olive oil as the substrate has been investigated. A noncompetitive inhibition by the surfactant on the enzyme was observed. Strong dependences of the kinetic constants k_cat and k_M, but not k_I on the water-to-surfactant ratio (R value) have been identified. The benefits of carrying out the hydrolysis at higher surfactant and water concentrations were demonstrated from the improvement of the initial rate and time course of conversion.

Original language	English
Pages (from-to)	89-98
Number of pages	10
Journal	Biocatalysis and Biotransformation
Volume	13
Issue number	2
DOIs	https://doi.org/10.3109/10242429509015215
State	Published - 1995
Externally published	Yes

Keywords

Hydrolysis
Lipase
Reverse micelles

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title = "Surfactant effects on lipase-catalyzed hydrolysis of olive oil in AOT/ISOOCTANE reverse micelles",

abstract = "The effects of surfactant concentration on the hydrolytic activity of Candida rugosa lipase in AOT/isooctane reverse micelles with olive oil as the substrate has been investigated. A noncompetitive inhibition by the surfactant on the enzyme was observed. Strong dependences of the kinetic constants kcat and kM, but not kI on the water-to-surfactant ratio (R value) have been identified. The benefits of carrying out the hydrolysis at higher surfactant and water concentrations were demonstrated from the improvement of the initial rate and time course of conversion.",

keywords = "Hydrolysis, Lipase, Reverse micelles",

author = "Tsai, {Shau Wei} and Lee, {Kung Ping} and Chiang, {Chen Li}",

year = "1995",

doi = "10.3109/10242429509015215",

language = "英语",

volume = "13",

pages = "89--98",

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TY - JOUR

T1 - Surfactant effects on lipase-catalyzed hydrolysis of olive oil in AOT/ISOOCTANE reverse micelles

AU - Tsai, Shau Wei

AU - Lee, Kung Ping

AU - Chiang, Chen Li

PY - 1995

Y1 - 1995

N2 - The effects of surfactant concentration on the hydrolytic activity of Candida rugosa lipase in AOT/isooctane reverse micelles with olive oil as the substrate has been investigated. A noncompetitive inhibition by the surfactant on the enzyme was observed. Strong dependences of the kinetic constants kcat and kM, but not kI on the water-to-surfactant ratio (R value) have been identified. The benefits of carrying out the hydrolysis at higher surfactant and water concentrations were demonstrated from the improvement of the initial rate and time course of conversion.

AB - The effects of surfactant concentration on the hydrolytic activity of Candida rugosa lipase in AOT/isooctane reverse micelles with olive oil as the substrate has been investigated. A noncompetitive inhibition by the surfactant on the enzyme was observed. Strong dependences of the kinetic constants kcat and kM, but not kI on the water-to-surfactant ratio (R value) have been identified. The benefits of carrying out the hydrolysis at higher surfactant and water concentrations were demonstrated from the improvement of the initial rate and time course of conversion.

KW - Hydrolysis

KW - Lipase

KW - Reverse micelles

UR - http://www.scopus.com/inward/record.url?scp=0029201693&partnerID=8YFLogxK

U2 - 10.3109/10242429509015215

DO - 10.3109/10242429509015215

M3 - 文章

AN - SCOPUS:0029201693

SN - 1024-2422

VL - 13

SP - 89

EP - 98

JO - Biocatalysis and Biotransformation

JF - Biocatalysis and Biotransformation

IS - 2

ER -

Surfactant effects on lipase-catalyzed hydrolysis of olive oil in AOT/ISOOCTANE reverse micelles

Abstract

Keywords

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