Synergistic regulation of human oxytocin receptor promoter by CCAAT/: Enhancer-binding protein and RELA

Shirin Khanjani*, Vasso Terzidou, Yun S. Lee, Steve Thornton, Mark R. Johnson, Phillip R. Bennett

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

10 Scopus citations

Abstract

Uterine activation is associated with increased oxytocin receptor (OXTR) expression and myometrial sensitivity to oxytocin. The OXTR promoter contains binding sites for CCAAT/enhancer-binding protein (CEBP) and nuclear factorkappa B p65 (RELA). RELA and CEBP beta (CEBPB) play a synergistic role in OXTR promoter activation. We created deletions in a DNA construct consisting of 850 bp upstream of the transcription start site linked to luc reporter to identify the CIS element of the OXTR promoter responsible for the synergistic activation by RELA and CEBPB. Deletion from -712 to -692 bp eliminated synergy, demonstrating that the critical region lies within these 20 bp. Binding studies showed that this sequence binds both RELA and CEBPB. The 20-bp critical region for synergistic activation of OXTR requires full-length RELA but only the basic leucine zipper domain of CEBPB.

Original languageEnglish
Pages (from-to)1083-1088
Number of pages6
JournalBiology of Reproduction
Volume85
Issue number5
DOIs
StatePublished - 01 11 2011
Externally publishedYes

Keywords

  • CEBPB
  • Cytokines
  • Gene regulation
  • Oxytocin
  • Oxytocin receptor (OXTR)
  • Parturition
  • Preterm labor
  • RELA

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