Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect

Ngoc Thi Thanh Tran; Tzu Hao Wang; Chiao Yun Lin; Yian Tai

doi:10.1016/j.bej.2013.04.017

Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect

Ngoc Thi Thanh Tran, Tzu Hao Wang, Chiao Yun Lin, Yian Tai^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

39 Scopus citations

Abstract

We have developed a simple approach to synthesize gold nanoparticle (AuNP) by utilizing the anti-cancer drug, methotrexate (MTX), as both the reducing agent and the capping molecule. MTX-conjugated AuNPs (MTX-AuNPs) of various sizes (~3 and ~20. nm) can be obtained in a one-pot synthesis. We utilized transmission electron microscopy, X-ray diffraction, and UV-vis spectroscopy to characterize these nanoparticles, and Fourier-transform infrared (FTIR) spectroscopy and Raman spectroscopy to investigate the chemical interactions between the MTX and AuNPs. To examine the cancer therapeutic effect of these nanoparticles, we applied the lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay tests. The results demonstrated that the MTX-AuNPs of small size (~3. nm) exhibited considerably high cytotoxic effects in human choriocarcinoma cell lines compared to the effects of equal doses of free MTX; these results suggest that anti-cancer drug-conjugated AuNPs have an enhanced therapeutic effect.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Biochemical Engineering Journal
Volume	78
DOIs	https://doi.org/10.1016/j.bej.2013.04.017
State	Published - 05 09 2013
Externally published	Yes

Keywords

Cancer therapy
Gold nanoparticles
In vitro
Methotrexate

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bej.2013.04.017

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@article{edfe2bb9db0049dbada16b74aedadba5,

title = "Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect",

abstract = "We have developed a simple approach to synthesize gold nanoparticle (AuNP) by utilizing the anti-cancer drug, methotrexate (MTX), as both the reducing agent and the capping molecule. MTX-conjugated AuNPs (MTX-AuNPs) of various sizes (\textasciitilde{}3 and \textasciitilde{}20. nm) can be obtained in a one-pot synthesis. We utilized transmission electron microscopy, X-ray diffraction, and UV-vis spectroscopy to characterize these nanoparticles, and Fourier-transform infrared (FTIR) spectroscopy and Raman spectroscopy to investigate the chemical interactions between the MTX and AuNPs. To examine the cancer therapeutic effect of these nanoparticles, we applied the lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay tests. The results demonstrated that the MTX-AuNPs of small size (\textasciitilde{}3. nm) exhibited considerably high cytotoxic effects in human choriocarcinoma cell lines compared to the effects of equal doses of free MTX; these results suggest that anti-cancer drug-conjugated AuNPs have an enhanced therapeutic effect.",

keywords = "Cancer therapy, Gold nanoparticles, In vitro, Methotrexate",

author = "Tran, \{Ngoc Thi Thanh\} and Wang, \{Tzu Hao\} and Lin, \{Chiao Yun\} and Yian Tai",

year = "2013",

month = sep,

day = "5",

doi = "10.1016/j.bej.2013.04.017",

language = "英语",

volume = "78",

pages = "175--180",

journal = "Biochemical Engineering Journal",

issn = "1369-703X",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect

AU - Tran, Ngoc Thi Thanh

AU - Wang, Tzu Hao

AU - Lin, Chiao Yun

AU - Tai, Yian

PY - 2013/9/5

Y1 - 2013/9/5

N2 - We have developed a simple approach to synthesize gold nanoparticle (AuNP) by utilizing the anti-cancer drug, methotrexate (MTX), as both the reducing agent and the capping molecule. MTX-conjugated AuNPs (MTX-AuNPs) of various sizes (~3 and ~20. nm) can be obtained in a one-pot synthesis. We utilized transmission electron microscopy, X-ray diffraction, and UV-vis spectroscopy to characterize these nanoparticles, and Fourier-transform infrared (FTIR) spectroscopy and Raman spectroscopy to investigate the chemical interactions between the MTX and AuNPs. To examine the cancer therapeutic effect of these nanoparticles, we applied the lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay tests. The results demonstrated that the MTX-AuNPs of small size (~3. nm) exhibited considerably high cytotoxic effects in human choriocarcinoma cell lines compared to the effects of equal doses of free MTX; these results suggest that anti-cancer drug-conjugated AuNPs have an enhanced therapeutic effect.

AB - We have developed a simple approach to synthesize gold nanoparticle (AuNP) by utilizing the anti-cancer drug, methotrexate (MTX), as both the reducing agent and the capping molecule. MTX-conjugated AuNPs (MTX-AuNPs) of various sizes (~3 and ~20. nm) can be obtained in a one-pot synthesis. We utilized transmission electron microscopy, X-ray diffraction, and UV-vis spectroscopy to characterize these nanoparticles, and Fourier-transform infrared (FTIR) spectroscopy and Raman spectroscopy to investigate the chemical interactions between the MTX and AuNPs. To examine the cancer therapeutic effect of these nanoparticles, we applied the lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay tests. The results demonstrated that the MTX-AuNPs of small size (~3. nm) exhibited considerably high cytotoxic effects in human choriocarcinoma cell lines compared to the effects of equal doses of free MTX; these results suggest that anti-cancer drug-conjugated AuNPs have an enhanced therapeutic effect.

KW - Cancer therapy

KW - Gold nanoparticles

KW - In vitro

KW - Methotrexate

UR - https://www.scopus.com/pages/publications/84882590963

U2 - 10.1016/j.bej.2013.04.017

DO - 10.1016/j.bej.2013.04.017

M3 - 文章

AN - SCOPUS:84882590963

SN - 1369-703X

VL - 78

SP - 175

EP - 180

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

ER -

Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect

Abstract

Keywords

UN SDGs

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