The combination effects of LiCL and the active leflunomide metabolite, A771726, on viral-induced interleukin 6 production and EV-A71 replication

Hui Chen Hung; Shin Ru Shih; Teng Yuan Chang; Ming Yu Fang; John T.A. Hsu

doi:10.1371/journal.pone.0111331

The combination effects of LiCL and the active leflunomide metabolite, A771726, on viral-induced interleukin 6 production and EV-A71 replication

Hui Chen Hung, Shin Ru Shih, Teng Yuan Chang, Ming Yu Fang, John T.A. Hsu

Department of Medical Biotechnology and Laboratory Science

Research output: Contribution to journal › Journal Article › peer-review

8 Scopus citations

Abstract

Enterovirus 71 (EV-A71) is a neurotropic virus that can cause severe complications involving the central nervous system. No effective antiviral therapeutics are available for treating EV-A71 infection and drug discovery efforts are rarely focused to target this disease. Thus, the main goal of this study was to discover existing drugs with novel indications that may effectively inhibit EV-A71 replication and the inflammatory cytokines elevation. In this study, we showed that LiCl, a GSK3b inhibitor, effectively suppressed EV-A71 replication, apoptosis and inflammatory cytokines production (Interleukin 6, Interleukin-1b) in infected cells. Furthermore, LiCl and an immunomodular agent were shown to strongly synergize with each other in suppressing EV-A71 replication. The results highlighted potential new treatment regimens in suppressing sequelae caused by EV-A71 replication. @copy; 2014 Hung et al.

Original language	English
Article number	e111331
Journal	PLoS ONE
Volume	9
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0111331
State	Published - 20 11 2014

Access to Document

10.1371/journal.pone.0111331

Cite this

@article{dee27ecde4044b75b2f86a8c773287f3,

title = "The combination effects of LiCL and the active leflunomide metabolite, A771726, on viral-induced interleukin 6 production and EV-A71 replication",

abstract = "Enterovirus 71 (EV-A71) is a neurotropic virus that can cause severe complications involving the central nervous system. No effective antiviral therapeutics are available for treating EV-A71 infection and drug discovery efforts are rarely focused to target this disease. Thus, the main goal of this study was to discover existing drugs with novel indications that may effectively inhibit EV-A71 replication and the inflammatory cytokines elevation. In this study, we showed that LiCl, a GSK3b inhibitor, effectively suppressed EV-A71 replication, apoptosis and inflammatory cytokines production (Interleukin 6, Interleukin-1b) in infected cells. Furthermore, LiCl and an immunomodular agent were shown to strongly synergize with each other in suppressing EV-A71 replication. The results highlighted potential new treatment regimens in suppressing sequelae caused by EV-A71 replication. @copy; 2014 Hung et al.",

author = "Hung, {Hui Chen} and Shih, {Shin Ru} and Chang, {Teng Yuan} and Fang, {Ming Yu} and Hsu, {John T.A.}",

year = "2014",

month = nov,

day = "20",

doi = "10.1371/journal.pone.0111331",

language = "英语",

volume = "9",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "11",

}

TY - JOUR

T1 - The combination effects of LiCL and the active leflunomide metabolite, A771726, on viral-induced interleukin 6 production and EV-A71 replication

AU - Hung, Hui Chen

AU - Shih, Shin Ru

AU - Chang, Teng Yuan

AU - Fang, Ming Yu

AU - Hsu, John T.A.

PY - 2014/11/20

Y1 - 2014/11/20

N2 - Enterovirus 71 (EV-A71) is a neurotropic virus that can cause severe complications involving the central nervous system. No effective antiviral therapeutics are available for treating EV-A71 infection and drug discovery efforts are rarely focused to target this disease. Thus, the main goal of this study was to discover existing drugs with novel indications that may effectively inhibit EV-A71 replication and the inflammatory cytokines elevation. In this study, we showed that LiCl, a GSK3b inhibitor, effectively suppressed EV-A71 replication, apoptosis and inflammatory cytokines production (Interleukin 6, Interleukin-1b) in infected cells. Furthermore, LiCl and an immunomodular agent were shown to strongly synergize with each other in suppressing EV-A71 replication. The results highlighted potential new treatment regimens in suppressing sequelae caused by EV-A71 replication. @copy; 2014 Hung et al.

AB - Enterovirus 71 (EV-A71) is a neurotropic virus that can cause severe complications involving the central nervous system. No effective antiviral therapeutics are available for treating EV-A71 infection and drug discovery efforts are rarely focused to target this disease. Thus, the main goal of this study was to discover existing drugs with novel indications that may effectively inhibit EV-A71 replication and the inflammatory cytokines elevation. In this study, we showed that LiCl, a GSK3b inhibitor, effectively suppressed EV-A71 replication, apoptosis and inflammatory cytokines production (Interleukin 6, Interleukin-1b) in infected cells. Furthermore, LiCl and an immunomodular agent were shown to strongly synergize with each other in suppressing EV-A71 replication. The results highlighted potential new treatment regimens in suppressing sequelae caused by EV-A71 replication. @copy; 2014 Hung et al.

UR - http://www.scopus.com/inward/record.url?scp=84912019914&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0111331

DO - 10.1371/journal.pone.0111331

M3 - 文章

C2 - 25412347

AN - SCOPUS:84912019914

SN - 1932-6203

VL - 9

JO - PLoS ONE

JF - PLoS ONE

IS - 11

M1 - e111331

ER -

The combination effects of LiCL and the active leflunomide metabolite, A771726, on viral-induced interleukin 6 production and EV-A71 replication

Abstract

Access to Document

Other files and links

Fingerprint

Cite this