The E3 ubiquitin ligase SIAH2 is a prosurvival factor overexpressed in oral cancer

Shu Chen Hsieh, Sheng Nan Kuo, Yi Han Zheng, Ming Hsui Tsai, Yu Sheng Lin, Ju Hwa Lin*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

12 Scopus citations


Background: This study aimed at investigating the expression and functional significance of seven in absentia homologues 2 (SIAH2), an E3 ubiquitin ligase, in oral squamous cell carcinoma (OSCC). Materials and Methods: Comparative genomic hybridization and real-time PCR were performed to examine the amplification of SIAH2 gene in clinical specimens of OSCC tissues. Expression of SIAH2 mRNA and protein was examined by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assays, respectively. Apoptosis was examined by annexing V staining and Poly (ADP-ribose) polymerase (PARP) cleavage, with or without siennamediated SIAH2 knockdown. The expression of p53 was analyzed by immunoblotting. Results: Levels of SIAH2 DNA and mRNA were significantly greater in clinical OSCC specimens and in cultured OSCC cells, which also stained positively for the SAIH2 protein. Knockdown of SIAH2 led to growth suppression and apoptosis induction in a p53- independent mechanism. Conclusion: These results revealed a tight correlation of SIAH2 overexpression with OSCC and suggest an oncogenic role of SIAH2 in oral cancer.

Original languageEnglish
Pages (from-to)4965-4974
Number of pages10
JournalAnticancer Research
Issue number11
StatePublished - 11 2013
Externally publishedYes


  • Apoptosis
  • Oral cancer
  • Siah2
  • Ubiquitin ligase


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