The effect of optically induced dielectrophoresis (ODEP)-based cell manipulation in a microfluidic system on the properties of biological cells

Po Yu Chu, Chia Hsun Hsieh, Chien Ru Lin, Min Hsien Wu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

23 Scopus citations

Abstract

Cell manipulation using optically induced dielectrophoresis (ODEP) in microfluidic systems has attracted the interest of scientists due to its simplicity. Although this technique has been successfully demonstrated for various applications, one fundamental issue has to be addressed-Whether, the ODEP field affects the native properties of cells. To address this issue, we explored the effect of ODEP electrical conditions on cellular properties. Within the experimental conditions tested, the ODEP-based cell manipulation with the largest velocity occurred at 10 Vpp and 1 MHz, for the two cancer cell types explored. Under this operating condition, however, the cell viability of cancer cells was significantly affected (e.g., 70.5 ± 10.0% and 50.6 ± 9.2% reduction for the PC-3 and SK-BR-3 cancer cells, respectively). Conversely, the exposure of cancer cells to the ODEP electrical conditions of 7-10 Vpp and 3-5 MHz did not significantly alter the cell viability, cell metabolic activity, and the EpCAM, VIM, and ABCC1 gene expression of cancer cells. Overall, this study fundamentally investigated the effect of ODEP electrical conditions on the cellular properties of cancer cells. The information obtained is crucially important for the utilization of ODEP-based cell manipulation in a microscale system for various applications.

Original languageEnglish
Article number65
JournalBiosensors
Volume10
Issue number6
DOIs
StatePublished - 06 2020

Bibliographical note

Publisher Copyright:
© 2020 by the authors.

Keywords

  • Cell manipulation
  • Cell physiology
  • Cell property
  • Microfluidic systems
  • Optically induced dielectrophoresis

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