The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy

Wei Wen Chen; Yung Hsiang Yi; Cheng Hao Chien; Kuei Ching Hsiung; Yi Chun Lin; Tian Hsiang Ma; Szecheng J. Lo; Ta Chau Chang

doi:10.1117/12.2210787

The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy

Wei Wen Chen, Yung Hsiang Yi, Cheng Hao Chien, Kuei Ching Hsiung, Yi Chun Lin, Tian Hsiang Ma, Szecheng J. Lo, Ta Chau Chang^*

^*Corresponding author for this work

Department of Biomedical Sciences (Master's Program in Clinical Trials Assessment)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Yolk lipoprotein constitutes the major source of energy and the materials for synthesizing signaling factors for the development of oocytes and embryos in C. elegans. Polyunsaturated fatty acids (PUFAs) packed in yolk lipoprotein have been recently recognized as critical molecules for fertilization and reproduction.¹ However, the relation between PUFAs and the homeostasis of yolk lipoprotein is not clear. Here we use coherent anti-Stokes Raman scattering (CARS) microscopy and two-photon excitation fluorescence (TPE-F) microscopy to examine the transportation of yolk lipoprotein. We demonstrate that CARS microscopy is a more sensitive method than the traditional Nile Red staining method in probing the abnormal accumulation of yolk lipoprotein in the body cavity of C. elegans. It is found that the accumulation of yolk lipoprotein is a time-dependent process. In addition, a negative correlation (r = -0.955) between reproductive aging and abnormal accumulation of yolk lipoprotein is established. We further examine wild-type, fat-1, and fat-2 worms with or without the expression of GFP-tagged yolk lipoprotein (VIT-2-GFP). Our data reveal that PUFAs have a positive effect on the synthesis and endocytosis of yolk lipoprotein, confirming the model proposed by Edmonds et al.²

Original language	English
Title of host publication	Optical Methods in Developmental Biology IV
Editors	Michael A. Choma, Scott E. Fraser, Andrew M. Rollins
Publisher	SPIE
ISBN (Electronic)	9781628419504
DOIs	https://doi.org/10.1117/12.2210787
State	Published - 2016
Event	Optical Methods in Developmental Biology IV - San Francisco, United States Duration: 13 02 2016 → 14 02 2016

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9716
ISSN (Print)	1605-7422

Conference

Conference	Optical Methods in Developmental Biology IV
Country/Territory	United States
City	San Francisco
Period	13/02/16 → 14/02/16

Bibliographical note

Publisher Copyright:
© 2016 SPIE.

Keywords

C. elegans
coherent Raman
polyunsaturated fatty acids
two-photon excitation fluorescence
yolk lipoprotein

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10.1117/12.2210787

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Chen, W. W., Yi, Y. H., Chien, C. H., Hsiung, K. C., Lin, Y. C., Ma, T. H., Lo, S. J., & Chang, T. C. (2016). The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy. In M. A. Choma, S. E. Fraser, & A. M. Rollins (Eds.), Optical Methods in Developmental Biology IV Article 97160G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9716). SPIE. https://doi.org/10.1117/12.2210787

Chen, Wei Wen ; Yi, Yung Hsiang ; Chien, Cheng Hao et al. / The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy. Optical Methods in Developmental Biology IV. editor / Michael A. Choma ; Scott E. Fraser ; Andrew M. Rollins. SPIE, 2016. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{245860236b9e47fa97affef077b8efa6,

title = "The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy",

abstract = "Yolk lipoprotein constitutes the major source of energy and the materials for synthesizing signaling factors for the development of oocytes and embryos in C. elegans. Polyunsaturated fatty acids (PUFAs) packed in yolk lipoprotein have been recently recognized as critical molecules for fertilization and reproduction.1 However, the relation between PUFAs and the homeostasis of yolk lipoprotein is not clear. Here we use coherent anti-Stokes Raman scattering (CARS) microscopy and two-photon excitation fluorescence (TPE-F) microscopy to examine the transportation of yolk lipoprotein. We demonstrate that CARS microscopy is a more sensitive method than the traditional Nile Red staining method in probing the abnormal accumulation of yolk lipoprotein in the body cavity of C. elegans. It is found that the accumulation of yolk lipoprotein is a time-dependent process. In addition, a negative correlation (r = -0.955) between reproductive aging and abnormal accumulation of yolk lipoprotein is established. We further examine wild-type, fat-1, and fat-2 worms with or without the expression of GFP-tagged yolk lipoprotein (VIT-2-GFP). Our data reveal that PUFAs have a positive effect on the synthesis and endocytosis of yolk lipoprotein, confirming the model proposed by Edmonds et al.2",

keywords = "C. elegans, coherent Raman, polyunsaturated fatty acids, two-photon excitation fluorescence, yolk lipoprotein",

author = "Chen, {Wei Wen} and Yi, {Yung Hsiang} and Chien, {Cheng Hao} and Hsiung, {Kuei Ching} and Lin, {Yi Chun} and Ma, {Tian Hsiang} and Lo, {Szecheng J.} and Chang, {Ta Chau}",

note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; Optical Methods in Developmental Biology IV ; Conference date: 13-02-2016 Through 14-02-2016",

year = "2016",

doi = "10.1117/12.2210787",

language = "英语",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Choma, {Michael A.} and Fraser, {Scott E.} and Rollins, {Andrew M.}",

booktitle = "Optical Methods in Developmental Biology IV",

address = "美国",

}

Chen, WW, Yi, YH, Chien, CH, Hsiung, KC, Lin, YC, Ma, TH, Lo, SJ & Chang, TC 2016, The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy. in MA Choma, SE Fraser & AM Rollins (eds), Optical Methods in Developmental Biology IV., 97160G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9716, SPIE, Optical Methods in Developmental Biology IV, San Francisco, United States, 13/02/16. https://doi.org/10.1117/12.2210787

The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy. / Chen, Wei Wen; Yi, Yung Hsiang; Chien, Cheng Hao et al.
Optical Methods in Developmental Biology IV. ed. / Michael A. Choma; Scott E. Fraser; Andrew M. Rollins. SPIE, 2016. 97160G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9716).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy

AU - Chen, Wei Wen

AU - Yi, Yung Hsiang

AU - Chien, Cheng Hao

AU - Hsiung, Kuei Ching

AU - Lin, Yi Chun

AU - Ma, Tian Hsiang

AU - Lo, Szecheng J.

AU - Chang, Ta Chau

PY - 2016

Y1 - 2016

N2 - Yolk lipoprotein constitutes the major source of energy and the materials for synthesizing signaling factors for the development of oocytes and embryos in C. elegans. Polyunsaturated fatty acids (PUFAs) packed in yolk lipoprotein have been recently recognized as critical molecules for fertilization and reproduction.1 However, the relation between PUFAs and the homeostasis of yolk lipoprotein is not clear. Here we use coherent anti-Stokes Raman scattering (CARS) microscopy and two-photon excitation fluorescence (TPE-F) microscopy to examine the transportation of yolk lipoprotein. We demonstrate that CARS microscopy is a more sensitive method than the traditional Nile Red staining method in probing the abnormal accumulation of yolk lipoprotein in the body cavity of C. elegans. It is found that the accumulation of yolk lipoprotein is a time-dependent process. In addition, a negative correlation (r = -0.955) between reproductive aging and abnormal accumulation of yolk lipoprotein is established. We further examine wild-type, fat-1, and fat-2 worms with or without the expression of GFP-tagged yolk lipoprotein (VIT-2-GFP). Our data reveal that PUFAs have a positive effect on the synthesis and endocytosis of yolk lipoprotein, confirming the model proposed by Edmonds et al.2

AB - Yolk lipoprotein constitutes the major source of energy and the materials for synthesizing signaling factors for the development of oocytes and embryos in C. elegans. Polyunsaturated fatty acids (PUFAs) packed in yolk lipoprotein have been recently recognized as critical molecules for fertilization and reproduction.1 However, the relation between PUFAs and the homeostasis of yolk lipoprotein is not clear. Here we use coherent anti-Stokes Raman scattering (CARS) microscopy and two-photon excitation fluorescence (TPE-F) microscopy to examine the transportation of yolk lipoprotein. We demonstrate that CARS microscopy is a more sensitive method than the traditional Nile Red staining method in probing the abnormal accumulation of yolk lipoprotein in the body cavity of C. elegans. It is found that the accumulation of yolk lipoprotein is a time-dependent process. In addition, a negative correlation (r = -0.955) between reproductive aging and abnormal accumulation of yolk lipoprotein is established. We further examine wild-type, fat-1, and fat-2 worms with or without the expression of GFP-tagged yolk lipoprotein (VIT-2-GFP). Our data reveal that PUFAs have a positive effect on the synthesis and endocytosis of yolk lipoprotein, confirming the model proposed by Edmonds et al.2

KW - C. elegans

KW - coherent Raman

KW - polyunsaturated fatty acids

KW - two-photon excitation fluorescence

KW - yolk lipoprotein

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U2 - 10.1117/12.2210787

DO - 10.1117/12.2210787

M3 - 会议稿件

AN - SCOPUS:84975086185

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Methods in Developmental Biology IV

A2 - Choma, Michael A.

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A2 - Rollins, Andrew M.

PB - SPIE

T2 - Optical Methods in Developmental Biology IV

Y2 - 13 February 2016 through 14 February 2016

ER -

Chen WW, Yi YH, Chien CH, Hsiung KC, Lin YC, Ma TH et al. The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy. In Choma MA, Fraser SE, Rollins AM, editors, Optical Methods in Developmental Biology IV. SPIE. 2016. 97160G. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2210787

The effect of polyunsaturated fatty acids on the homeostasis of yolk lipoprotein in C. elegans examined by CARS and two-photon excitation fluorescence (TPE-F) microscopy

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