TY - JOUR
T1 - The effects of different thawing rates on porcine corneal croypreservation
AU - Lu, Paul C.S.
AU - Tsai, Ray J.F.
AU - Lin, May S.M.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose. To compare the effects of different thawing rates on the viability of porcine corneas after cryopreservation. Hypothesis. The slow thawing rate, same as cooling rate, will make less damage to the cornea after cryopreservation. Methods. Each group containing 2 pig corneas, with cooling rate, -1°C/min. All groups, except Gr1., use minimal 1M DMSO in CPTES. Gr1. Frozen in air without DMSO, thawing rate 1 °C/min. Gr2. Frozen in air, thawing rate 1 °C/min. Gr3. Frozen in air, thawing rate 5 °C /min. Gr4. Frozen in air, thawing rate 10 °C/min. Gr5. Frozen in air, quick thawing (37 °C water bath, 3-4 min). Combined staining with alizarine red-S and trypane blue. Statistical analysis: One-Way ANOVA analysis. Results. Among the five groups, the result of Gr2. has statistically significant difference (P< 0.0001) to any other group. Gr4.(10 °C/min) and Gr5.(37 °C /min) have no statistical differences. Conclusions. The slow thawing rate (1 °C/min) does the least damage to the cryopreserved corneas. The same slow thawing rate and cooling rate may create a reversible condition, which could preserve most corneal endothelial cells in this study. The thawing rate greater than 10 °C/min will do much damage to corneal endothelial cells.
AB - Purpose. To compare the effects of different thawing rates on the viability of porcine corneas after cryopreservation. Hypothesis. The slow thawing rate, same as cooling rate, will make less damage to the cornea after cryopreservation. Methods. Each group containing 2 pig corneas, with cooling rate, -1°C/min. All groups, except Gr1., use minimal 1M DMSO in CPTES. Gr1. Frozen in air without DMSO, thawing rate 1 °C/min. Gr2. Frozen in air, thawing rate 1 °C/min. Gr3. Frozen in air, thawing rate 5 °C /min. Gr4. Frozen in air, thawing rate 10 °C/min. Gr5. Frozen in air, quick thawing (37 °C water bath, 3-4 min). Combined staining with alizarine red-S and trypane blue. Statistical analysis: One-Way ANOVA analysis. Results. Among the five groups, the result of Gr2. has statistically significant difference (P< 0.0001) to any other group. Gr4.(10 °C/min) and Gr5.(37 °C /min) have no statistical differences. Conclusions. The slow thawing rate (1 °C/min) does the least damage to the cryopreserved corneas. The same slow thawing rate and cooling rate may create a reversible condition, which could preserve most corneal endothelial cells in this study. The thawing rate greater than 10 °C/min will do much damage to corneal endothelial cells.
UR - https://www.scopus.com/pages/publications/33750190382
M3 - 文章
AN - SCOPUS:33750190382
SN - 0146-0404
VL - 37
SP - S84
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 3
ER -