The enhancer sequence of human hepatitis B virus can enhance the activity of its surface gene promoter

Hsiao Kuey Chang; Chen Kung Chou; Chungming Chang; Tsung Sheng Su; Cheng Po Hu; Mitsuaki Yoshida; Ling Pal Ting

doi:10.1093/nar/15.5.2261

The enhancer sequence of human hepatitis B virus can enhance the activity of its surface gene promoter

Hsiao Kuey Chang, Chen Kung Chou, Chungming Chang, Tsung Sheng Su, Cheng Po Hu, Mitsuaki Yoshida, Ling Pal Ting^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

27 Scopus citations

Abstract

The transcrlptlonal enhancer sequence has recently been demonstrated in the hepatitis B viral genome. This enhancer sequence has also been shown to Increase the activity of HBcAg gene promoter. Using the chloramphenlcol acetyltransferase gene expression system, we demonstrated that the Intrinsic promoter activity of HBsAg gene promoter was stronger than that of HBcAg gene promoter in both human hepatoma cell lines, Hep3B and HuH-7. Furthermore, we showed that the HBV enhancer sequence not only stimulated the EBcAg gene promoter activity, but also stimulated the RBsAg gene promoter activity in both Hep3B and HuH-7 cells. The enhancer sequence Increased the HBsAg gene promoter activity 20-fold In both Hep3B and HuH-7 cell lines, while the increase of the HBcAg gene promoter was 2- and 8-fold in Hep3B and HuH-7 cells, respectively.

Original language	English
Pages (from-to)	2261-2268
Number of pages	8
Journal	Nucleic Acids Research
Volume	15
Issue number	5
DOIs	https://doi.org/10.1093/nar/15.5.2261
State	Published - 15 03 1987
Externally published	Yes

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title = "The enhancer sequence of human hepatitis B virus can enhance the activity of its surface gene promoter",

abstract = "The transcrlptlonal enhancer sequence has recently been demonstrated in the hepatitis B viral genome. This enhancer sequence has also been shown to Increase the activity of HBcAg gene promoter. Using the chloramphenlcol acetyltransferase gene expression system, we demonstrated that the Intrinsic promoter activity of HBsAg gene promoter was stronger than that of HBcAg gene promoter in both human hepatoma cell lines, Hep3B and HuH-7. Furthermore, we showed that the HBV enhancer sequence not only stimulated the EBcAg gene promoter activity, but also stimulated the RBsAg gene promoter activity in both Hep3B and HuH-7 cells. The enhancer sequence Increased the HBsAg gene promoter activity 20-fold In both Hep3B and HuH-7 cell lines, while the increase of the HBcAg gene promoter was 2- and 8-fold in Hep3B and HuH-7 cells, respectively.",

author = "Chang, {Hsiao Kuey} and Chou, {Chen Kung} and Chungming Chang and Su, {Tsung Sheng} and Hu, {Cheng Po} and Mitsuaki Yoshida and Ting, {Ling Pal}",

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T1 - The enhancer sequence of human hepatitis B virus can enhance the activity of its surface gene promoter

AU - Chang, Hsiao Kuey

AU - Chou, Chen Kung

AU - Chang, Chungming

AU - Su, Tsung Sheng

AU - Hu, Cheng Po

AU - Yoshida, Mitsuaki

AU - Ting, Ling Pal

PY - 1987/3/15

Y1 - 1987/3/15

N2 - The transcrlptlonal enhancer sequence has recently been demonstrated in the hepatitis B viral genome. This enhancer sequence has also been shown to Increase the activity of HBcAg gene promoter. Using the chloramphenlcol acetyltransferase gene expression system, we demonstrated that the Intrinsic promoter activity of HBsAg gene promoter was stronger than that of HBcAg gene promoter in both human hepatoma cell lines, Hep3B and HuH-7. Furthermore, we showed that the HBV enhancer sequence not only stimulated the EBcAg gene promoter activity, but also stimulated the RBsAg gene promoter activity in both Hep3B and HuH-7 cells. The enhancer sequence Increased the HBsAg gene promoter activity 20-fold In both Hep3B and HuH-7 cell lines, while the increase of the HBcAg gene promoter was 2- and 8-fold in Hep3B and HuH-7 cells, respectively.

AB - The transcrlptlonal enhancer sequence has recently been demonstrated in the hepatitis B viral genome. This enhancer sequence has also been shown to Increase the activity of HBcAg gene promoter. Using the chloramphenlcol acetyltransferase gene expression system, we demonstrated that the Intrinsic promoter activity of HBsAg gene promoter was stronger than that of HBcAg gene promoter in both human hepatoma cell lines, Hep3B and HuH-7. Furthermore, we showed that the HBV enhancer sequence not only stimulated the EBcAg gene promoter activity, but also stimulated the RBsAg gene promoter activity in both Hep3B and HuH-7 cells. The enhancer sequence Increased the HBsAg gene promoter activity 20-fold In both Hep3B and HuH-7 cell lines, while the increase of the HBcAg gene promoter was 2- and 8-fold in Hep3B and HuH-7 cells, respectively.

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The enhancer sequence of human hepatitis B virus can enhance the activity of its surface gene promoter

Abstract

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