The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis

Pei Shan Yang; Yu-Chao Hsu; Yu Hsiang Lin; Cheng Pang Hou; Chien Lun Chen; Phei-Lang Chang; Horng Heng Juang; Ke-Hung Tsui

doi:10.2147/OTT.S69239

The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis

Pei Shan Yang, Yu-Chao Hsu, Yu Hsiang Lin, Cheng Pang Hou, Chien Lun Chen, Phei-Lang Chang, Horng Heng Juang, Ke-Hung Tsui^*

^*Corresponding author for this work

Department of Anatomy

Research output: Contribution to journal › Journal Article › peer-review

2 Scopus citations

Abstract

Patients and methods: From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade bladder carcinoma and five with low-grade bladder carcinoma. ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4). Immunohistochemistry and real-time reverse transcription-polymerase chain reaction were used to validate the degree of EST expression and the prognostic value of ESTs.

Results: Apoferritin H subunit (FTH1 protein) exhibited increased expression in high-grade bladder carcinoma compared with that seen in low-grade carcinoma. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction both supported the higher expression of FTH1 in high-grade urothelial carcinoma.

Conclusion: ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.

Purpose: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.

Original language	English
Pages (from-to)	1777-1781
Number of pages	5
Journal	OncoTargets and Therapy
Volume	7
DOIs	https://doi.org/10.2147/OTT.S69239
State	Published - 30 09 2014

Bibliographical note

Publisher Copyright:
© 2014 Yang et al.

Keywords

Expression sequence tag
FTH1
Transitional cell carcinoma

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title = "The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis",

abstract = "Patients and methods: From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade bladder carcinoma and five with low-grade bladder carcinoma. ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4). Immunohistochemistry and real-time reverse transcription-polymerase chain reaction were used to validate the degree of EST expression and the prognostic value of ESTs.Results: Apoferritin H subunit (FTH1 protein) exhibited increased expression in high-grade bladder carcinoma compared with that seen in low-grade carcinoma. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction both supported the higher expression of FTH1 in high-grade urothelial carcinoma.Conclusion: ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.Purpose: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.",

keywords = "Expression sequence tag, FTH1, Transitional cell carcinoma",

author = "Yang, {Pei Shan} and Yu-Chao Hsu and Lin, {Yu Hsiang} and Hou, {Cheng Pang} and Chen, {Chien Lun} and Phei-Lang Chang and Juang, {Horng Heng} and Ke-Hung Tsui",

note = "Publisher Copyright: {\textcopyright} 2014 Yang et al.",

year = "2014",

month = sep,

day = "30",

doi = "10.2147/OTT.S69239",

language = "英语",

volume = "7",

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T1 - The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis

AU - Yang, Pei Shan

AU - Hsu, Yu-Chao

AU - Lin, Yu Hsiang

AU - Hou, Cheng Pang

AU - Chen, Chien Lun

AU - Chang, Phei-Lang

AU - Juang, Horng Heng

AU - Tsui, Ke-Hung

PY - 2014/9/30

Y1 - 2014/9/30

N2 - Patients and methods: From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade bladder carcinoma and five with low-grade bladder carcinoma. ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4). Immunohistochemistry and real-time reverse transcription-polymerase chain reaction were used to validate the degree of EST expression and the prognostic value of ESTs.Results: Apoferritin H subunit (FTH1 protein) exhibited increased expression in high-grade bladder carcinoma compared with that seen in low-grade carcinoma. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction both supported the higher expression of FTH1 in high-grade urothelial carcinoma.Conclusion: ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.Purpose: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.

AB - Patients and methods: From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade bladder carcinoma and five with low-grade bladder carcinoma. ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4). Immunohistochemistry and real-time reverse transcription-polymerase chain reaction were used to validate the degree of EST expression and the prognostic value of ESTs.Results: Apoferritin H subunit (FTH1 protein) exhibited increased expression in high-grade bladder carcinoma compared with that seen in low-grade carcinoma. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction both supported the higher expression of FTH1 in high-grade urothelial carcinoma.Conclusion: ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.Purpose: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.

KW - Expression sequence tag

KW - FTH1

KW - Transitional cell carcinoma

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DO - 10.2147/OTT.S69239

M3 - 文章

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SN - 1178-6930

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JO - OncoTargets and Therapy

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ER -

The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis

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