TY - JOUR
T1 - The follicular dendritic cell restricted epitope, FDC-M2, is complement C4; localization of immune complexes in mouse tissues
AU - Taylor, Philip R.
AU - Pickering, Matthew C.
AU - Kosco-Vilbois, Marie H.
AU - Walport, Mark J.
AU - Botto, Marina
AU - Gordon, Siamon
AU - Martinez-Pomares, Luisa
PY - 2002
Y1 - 2002
N2 - We have identified the murine follicular dendritic cell (FDC) marker, FDC-M2, recognized by monoclonal antibody mAb209, as complement component C4. Consistent with this, FDC-M2 was detectable at sites of immune complex-mediated inflammatory disease. Analysis of FDC-M2 distribution in complement-deficient mice highlighted the differences in immune complex clearance between these mice, and showed that a population of uncharacterized FDC-M2+ reticular and perivascular cells in the spleen, distinct from FDC, are also involved in immune complex capture and possibly retention. These results demonstrate that mAb209, in addition to its role as an FDC marker, is a valuable reagent for the analysis of complement deposition in vivo.
AB - We have identified the murine follicular dendritic cell (FDC) marker, FDC-M2, recognized by monoclonal antibody mAb209, as complement component C4. Consistent with this, FDC-M2 was detectable at sites of immune complex-mediated inflammatory disease. Analysis of FDC-M2 distribution in complement-deficient mice highlighted the differences in immune complex clearance between these mice, and showed that a population of uncharacterized FDC-M2+ reticular and perivascular cells in the spleen, distinct from FDC, are also involved in immune complex capture and possibly retention. These results demonstrate that mAb209, in addition to its role as an FDC marker, is a valuable reagent for the analysis of complement deposition in vivo.
KW - Complement
KW - Knockout
KW - Lymph node
KW - Rodent
KW - Spleen
KW - Transgenic
UR - http://www.scopus.com/inward/record.url?scp=0036318879&partnerID=8YFLogxK
U2 - 10.1002/1521-4141(200207)32:7<1883::aid-immu1888>3.0.co;2-8
DO - 10.1002/1521-4141(200207)32:7<1883::aid-immu1888>3.0.co;2-8
M3 - 文章
C2 - 12115608
AN - SCOPUS:0036318879
SN - 0014-2980
VL - 32
SP - 1883
EP - 1896
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 7
ER -