The gelling effect of platelet-rich fibrin matrix when exposed to human tenocytes from the rotator cuff in small-diameter culture wells and the design of a co-culture device to overcome this phenomenon

Objectives platelet-rich fibrin matrix (pRFM) has been proved to enhance tenocyte proliferation but has mixed results when used during rotator cuff repair. The optimal pRFM preparation protocol should be determined before clinical application. To screen the best pRFM to each individual's tenocytes effectively, small-diameter culture wells should be used to increase variables. The gelling effect of pRFM will occur when small-diameter culture wells are used. A co-culture device should be designed to avoid this effect. Methods Tenocytes harvested during rotator cuff repair and blood from a healthy volunteer were used. Tenocytes were seeded in 96-, 24-, 12-, and six-well plates and co-culture devices. Appropriate volumes of pRFM, according to the surface area of each culture well, were treated with tenocytes for seven days. The co-culture device was designed to avoid the gelling effect that occurred in the small-diameter culture well. cell proliferation was analyzed by water soluble tetrazolium-1 (WsT-1) bioassay. Results the relative quantification (condition/control) of WsT-1 assay on day seven revealed a significant decrease in tenocyte proliferation in small-diameter culture wells (96 and 24 wells) due to the gelling effect. pRFM in large-diameter culture wells (12 and six wells) and co-culture systems induced a significant increase in tenocyte proliferation compared with the control group. The gelling effect of pRFM was avoided by the co-culture device. Conclusion When pRFM and tenocytes are cultured in small-diameter culture wells, the gelling effect will occur and make screening of personalized best-fit pRFM difficult. This effect can be avoided with the co-culture device.