The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis

Po Ling Chang; Wen Shyang Hsieh; Chia Lien Chiang; Marion J. Tuohy; Gerri S. Hall; Gary W. Procop; Huan Tsung Chang; Hsin Tsung Ho

doi:10.1016/j.diagmicrobio.2007.02.004

The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis

Po Ling Chang
, Wen Shyang Hsieh
, Chia Lien Chiang
, Marion J. Tuohy
, Gerri S. Hall
, Gary W. Procop
, Huan Tsung Chang^*
, Hsin Tsung Ho

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

20 Scopus citations

Abstract

To rapidly identify Mycobacterium and Nocardia spp. without costly probes, we had implemented capillary electrophoresis (CE) in polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis to analyze their 65-kDa heat shock protein (hsp65) gene. The PCR-RFLP analysis with CE (PRACE) involved only one restriction enzyme, HaeIII, and a single electrophoretic separation less than 10 min. Full-range (10-200 bp) RFLP patterns of 12 less common Mycobacterium and 7 Nocardia spp. were investigated. A good agreement was observed between the sizes of restriction fragments resolved by CE and the real sizes deduced from sequence analysis. Including hsp65 gene patterns of 12 Mycobacterium spp. published earlier, differentiation was distinct among 24 Mycobacterium and 7 Nocardia spp. Some closely related species exhibiting similar biochemical characteristics could be well discriminated by an extra HaeIII digestion site. Thus, PRACE offers a nonprobe alternative for rapid identification of various cultured Mycobacterium and Nocardia to the species level.

Original language	English
Pages (from-to)	315-323
Number of pages	9
Journal	Diagnostic Microbiology and Infectious Disease
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2007.02.004
State	Published - 07 2007
Externally published	Yes

Keywords

Capillary electrophoresis
Mycobacteria other than tuberculosis
Nocardia
PCR-RFLP analysis
Restriction fragment length polymorphism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2007.02.004

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Chang, P. L., Hsieh, W. S., Chiang, C. L., Tuohy, M. J., Hall, G. S., Procop, G. W., Chang, H. T., & Ho, H. T. (2007). The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis. Diagnostic Microbiology and Infectious Disease, 58(3), 315-323. https://doi.org/10.1016/j.diagmicrobio.2007.02.004

@article{1af359fbda544530a7a642ae1c46502f,

title = "The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis",

abstract = "To rapidly identify Mycobacterium and Nocardia spp. without costly probes, we had implemented capillary electrophoresis (CE) in polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis to analyze their 65-kDa heat shock protein (hsp65) gene. The PCR-RFLP analysis with CE (PRACE) involved only one restriction enzyme, HaeIII, and a single electrophoretic separation less than 10 min. Full-range (10-200 bp) RFLP patterns of 12 less common Mycobacterium and 7 Nocardia spp. were investigated. A good agreement was observed between the sizes of restriction fragments resolved by CE and the real sizes deduced from sequence analysis. Including hsp65 gene patterns of 12 Mycobacterium spp. published earlier, differentiation was distinct among 24 Mycobacterium and 7 Nocardia spp. Some closely related species exhibiting similar biochemical characteristics could be well discriminated by an extra HaeIII digestion site. Thus, PRACE offers a nonprobe alternative for rapid identification of various cultured Mycobacterium and Nocardia to the species level.",

keywords = "Capillary electrophoresis, Mycobacteria other than tuberculosis, Nocardia, PCR-RFLP analysis, Restriction fragment length polymorphism",

author = "Chang, \{Po Ling\} and Hsieh, \{Wen Shyang\} and Chiang, \{Chia Lien\} and Tuohy, \{Marion J.\} and Hall, \{Gerri S.\} and Procop, \{Gary W.\} and Chang, \{Huan Tsung\} and Ho, \{Hsin Tsung\}",

year = "2007",

month = jul,

doi = "10.1016/j.diagmicrobio.2007.02.004",

language = "英语",

volume = "58",

pages = "315--323",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "3",

}

Chang, PL, Hsieh, WS, Chiang, CL, Tuohy, MJ, Hall, GS, Procop, GW, Chang, HT & Ho, HT 2007, 'The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis', Diagnostic Microbiology and Infectious Disease, vol. 58, no. 3, pp. 315-323. https://doi.org/10.1016/j.diagmicrobio.2007.02.004

The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis. / Chang, Po Ling; Hsieh, Wen Shyang; Chiang, Chia Lien et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 58, No. 3, 07.2007, p. 315-323.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis

AU - Chang, Po Ling

AU - Hsieh, Wen Shyang

AU - Chiang, Chia Lien

AU - Tuohy, Marion J.

AU - Hall, Gerri S.

AU - Procop, Gary W.

AU - Chang, Huan Tsung

AU - Ho, Hsin Tsung

PY - 2007/7

Y1 - 2007/7

N2 - To rapidly identify Mycobacterium and Nocardia spp. without costly probes, we had implemented capillary electrophoresis (CE) in polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis to analyze their 65-kDa heat shock protein (hsp65) gene. The PCR-RFLP analysis with CE (PRACE) involved only one restriction enzyme, HaeIII, and a single electrophoretic separation less than 10 min. Full-range (10-200 bp) RFLP patterns of 12 less common Mycobacterium and 7 Nocardia spp. were investigated. A good agreement was observed between the sizes of restriction fragments resolved by CE and the real sizes deduced from sequence analysis. Including hsp65 gene patterns of 12 Mycobacterium spp. published earlier, differentiation was distinct among 24 Mycobacterium and 7 Nocardia spp. Some closely related species exhibiting similar biochemical characteristics could be well discriminated by an extra HaeIII digestion site. Thus, PRACE offers a nonprobe alternative for rapid identification of various cultured Mycobacterium and Nocardia to the species level.

AB - To rapidly identify Mycobacterium and Nocardia spp. without costly probes, we had implemented capillary electrophoresis (CE) in polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis to analyze their 65-kDa heat shock protein (hsp65) gene. The PCR-RFLP analysis with CE (PRACE) involved only one restriction enzyme, HaeIII, and a single electrophoretic separation less than 10 min. Full-range (10-200 bp) RFLP patterns of 12 less common Mycobacterium and 7 Nocardia spp. were investigated. A good agreement was observed between the sizes of restriction fragments resolved by CE and the real sizes deduced from sequence analysis. Including hsp65 gene patterns of 12 Mycobacterium spp. published earlier, differentiation was distinct among 24 Mycobacterium and 7 Nocardia spp. Some closely related species exhibiting similar biochemical characteristics could be well discriminated by an extra HaeIII digestion site. Thus, PRACE offers a nonprobe alternative for rapid identification of various cultured Mycobacterium and Nocardia to the species level.

KW - Capillary electrophoresis

KW - Mycobacteria other than tuberculosis

KW - Nocardia

KW - PCR-RFLP analysis

KW - Restriction fragment length polymorphism

UR - https://www.scopus.com/pages/publications/34347358396

U2 - 10.1016/j.diagmicrobio.2007.02.004

DO - 10.1016/j.diagmicrobio.2007.02.004

M3 - 文章

C2 - 17382507

AN - SCOPUS:34347358396

SN - 0732-8893

VL - 58

SP - 315

EP - 323

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis

Abstract

Keywords

UN SDGs

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