The Impacts of Lymph on the Adipogenesis of Adipose-Derived Stem Cells

Hui Yi Hsiao; Jia Wei Liu; Marco Pappalardo; Ming Huei Cheng

doi:10.1097/PRS.0000000000010082

The Impacts of Lymph on the Adipogenesis of Adipose-Derived Stem Cells

Hui Yi Hsiao, Jia Wei Liu, Marco Pappalardo, Ming Huei Cheng^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

2 Scopus citations

Abstract

BACKGROUND: The pathophysiology of adipose proliferation or differentiation in extremity lymphedema has not been thoroughly studied. This study investigated the impacts of the lymph harvested from lymphedematous limbs on the adipogenesis of adipose-derived stem cells (ASCs).

METHODS: ASCs were isolated from the adipose tissue of normal extremities and cultured with lymph collected from Cheng lymphedema grade III to IV patients or adipogenic differentiation medium (ADM) and further subjected to differentiation and proliferation assay. The expression of adipogenesis genes was examined by real-time polymerase chain reaction to investigate the effect of lymph on ASCs. The level of adipogenic cytokines in the lymph was also evaluated.

RESULTS: The adipocytes were significantly larger in lymphedema fat tissue compared with that in normal fat tissues ( P < 0.00). The adipogenesis of ASCs cultured in lymph was significantly enhanced compared with in ADM ( P = 0.008) on day 10, suggesting that the adipogenesis of ASCs was promoted under the lymph-cultured environment. The expression of adipogenesis genes, peroxisome proliferator-activated receptor ( P = 0.02), CAAT/enhancer-binding protein α ( P = 0.008); fatty-acid binding protein ( P = 0.004), and lipoprotein lipase ( P = 0.003), was statistically elevated when the ASCs were cultured with lymph. The insulin content in lymph was statistically higher in lymph ( P < 0.001) than in plasma.

CONCLUSIONS: The adipogenesis of ASCs was promoted under the lymph-cultured environment with statistically increased adipogenesis genes of peroxisome proliferator-activated receptor, CAAT/enhancer-binding protein α, fatty-acid binding protein, and lipoprotein lipase. The excess lymph accumulated in the lymphedematous extremity contained a greater insulin/insulin-like growth factor-2. These adipogenic factors promoted the expression of early adipogenesis genes and led ASCs to undergo adipogenesis and differentiated into adipocytes.

CLINICAL RELEVANCE STATEMENT: The accumulation of adipose tissue in the lymphedema region was contributed from the content of excess lymph.

Original language	English
Pages (from-to)	1005-1015
Number of pages	11
Journal	Plastic and Reconstructive Surgery
Volume	151
Issue number	5
DOIs	https://doi.org/10.1097/PRS.0000000000010082
State	Published - 01 05 2023
Externally published	Yes

Bibliographical note

Keywords

Humans
Adipogenesis/physiology
Lipoprotein Lipase/metabolism
Peroxisome Proliferator-Activated Receptors/metabolism
Adipocytes/physiology
Adipose Tissue
Cell Differentiation/genetics
Stem Cells/physiology
Lymphedema
Insulins/metabolism
Cells, Cultured

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10.1097/PRS.0000000000010082

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@article{922690269d5f443e9f6bc14667dbcdad,

title = "The Impacts of Lymph on the Adipogenesis of Adipose-Derived Stem Cells",

abstract = "BACKGROUND: The pathophysiology of adipose proliferation or differentiation in extremity lymphedema has not been thoroughly studied. This study investigated the impacts of the lymph harvested from lymphedematous limbs on the adipogenesis of adipose-derived stem cells (ASCs).METHODS: ASCs were isolated from the adipose tissue of normal extremities and cultured with lymph collected from Cheng lymphedema grade III to IV patients or adipogenic differentiation medium (ADM) and further subjected to differentiation and proliferation assay. The expression of adipogenesis genes was examined by real-time polymerase chain reaction to investigate the effect of lymph on ASCs. The level of adipogenic cytokines in the lymph was also evaluated.RESULTS: The adipocytes were significantly larger in lymphedema fat tissue compared with that in normal fat tissues ( P < 0.00). The adipogenesis of ASCs cultured in lymph was significantly enhanced compared with in ADM ( P = 0.008) on day 10, suggesting that the adipogenesis of ASCs was promoted under the lymph-cultured environment. The expression of adipogenesis genes, peroxisome proliferator-activated receptor ( P = 0.02), CAAT/enhancer-binding protein α ( P = 0.008); fatty-acid binding protein ( P = 0.004), and lipoprotein lipase ( P = 0.003), was statistically elevated when the ASCs were cultured with lymph. The insulin content in lymph was statistically higher in lymph ( P < 0.001) than in plasma.CONCLUSIONS: The adipogenesis of ASCs was promoted under the lymph-cultured environment with statistically increased adipogenesis genes of peroxisome proliferator-activated receptor, CAAT/enhancer-binding protein α, fatty-acid binding protein, and lipoprotein lipase. The excess lymph accumulated in the lymphedematous extremity contained a greater insulin/insulin-like growth factor-2. These adipogenic factors promoted the expression of early adipogenesis genes and led ASCs to undergo adipogenesis and differentiated into adipocytes.CLINICAL RELEVANCE STATEMENT: The accumulation of adipose tissue in the lymphedema region was contributed from the content of excess lymph.",

keywords = "Humans, Adipogenesis/physiology, Lipoprotein Lipase/metabolism, Peroxisome Proliferator-Activated Receptors/metabolism, Adipocytes/physiology, Adipose Tissue, Cell Differentiation/genetics, Stem Cells/physiology, Lymphedema, Insulins/metabolism, Cells, Cultured",

author = "Hsiao, {Hui Yi} and Liu, {Jia Wei} and Marco Pappalardo and Cheng, {Ming Huei}",

note = "Copyright {\textcopyright} 2022 by the American Society of Plastic Surgeons.",

year = "2023",

month = may,

day = "1",

doi = "10.1097/PRS.0000000000010082",

language = "英语",

volume = "151",

pages = "1005--1015",

journal = "Plastic and Reconstructive Surgery",

issn = "0032-1052",

publisher = "Lippincott Williams and Wilkins",

number = "5",

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T1 - The Impacts of Lymph on the Adipogenesis of Adipose-Derived Stem Cells

AU - Hsiao, Hui Yi

AU - Liu, Jia Wei

AU - Pappalardo, Marco

AU - Cheng, Ming Huei

PY - 2023/5/1

Y1 - 2023/5/1

N2 - BACKGROUND: The pathophysiology of adipose proliferation or differentiation in extremity lymphedema has not been thoroughly studied. This study investigated the impacts of the lymph harvested from lymphedematous limbs on the adipogenesis of adipose-derived stem cells (ASCs).METHODS: ASCs were isolated from the adipose tissue of normal extremities and cultured with lymph collected from Cheng lymphedema grade III to IV patients or adipogenic differentiation medium (ADM) and further subjected to differentiation and proliferation assay. The expression of adipogenesis genes was examined by real-time polymerase chain reaction to investigate the effect of lymph on ASCs. The level of adipogenic cytokines in the lymph was also evaluated.RESULTS: The adipocytes were significantly larger in lymphedema fat tissue compared with that in normal fat tissues ( P < 0.00). The adipogenesis of ASCs cultured in lymph was significantly enhanced compared with in ADM ( P = 0.008) on day 10, suggesting that the adipogenesis of ASCs was promoted under the lymph-cultured environment. The expression of adipogenesis genes, peroxisome proliferator-activated receptor ( P = 0.02), CAAT/enhancer-binding protein α ( P = 0.008); fatty-acid binding protein ( P = 0.004), and lipoprotein lipase ( P = 0.003), was statistically elevated when the ASCs were cultured with lymph. The insulin content in lymph was statistically higher in lymph ( P < 0.001) than in plasma.CONCLUSIONS: The adipogenesis of ASCs was promoted under the lymph-cultured environment with statistically increased adipogenesis genes of peroxisome proliferator-activated receptor, CAAT/enhancer-binding protein α, fatty-acid binding protein, and lipoprotein lipase. The excess lymph accumulated in the lymphedematous extremity contained a greater insulin/insulin-like growth factor-2. These adipogenic factors promoted the expression of early adipogenesis genes and led ASCs to undergo adipogenesis and differentiated into adipocytes.CLINICAL RELEVANCE STATEMENT: The accumulation of adipose tissue in the lymphedema region was contributed from the content of excess lymph.

AB - BACKGROUND: The pathophysiology of adipose proliferation or differentiation in extremity lymphedema has not been thoroughly studied. This study investigated the impacts of the lymph harvested from lymphedematous limbs on the adipogenesis of adipose-derived stem cells (ASCs).METHODS: ASCs were isolated from the adipose tissue of normal extremities and cultured with lymph collected from Cheng lymphedema grade III to IV patients or adipogenic differentiation medium (ADM) and further subjected to differentiation and proliferation assay. The expression of adipogenesis genes was examined by real-time polymerase chain reaction to investigate the effect of lymph on ASCs. The level of adipogenic cytokines in the lymph was also evaluated.RESULTS: The adipocytes were significantly larger in lymphedema fat tissue compared with that in normal fat tissues ( P < 0.00). The adipogenesis of ASCs cultured in lymph was significantly enhanced compared with in ADM ( P = 0.008) on day 10, suggesting that the adipogenesis of ASCs was promoted under the lymph-cultured environment. The expression of adipogenesis genes, peroxisome proliferator-activated receptor ( P = 0.02), CAAT/enhancer-binding protein α ( P = 0.008); fatty-acid binding protein ( P = 0.004), and lipoprotein lipase ( P = 0.003), was statistically elevated when the ASCs were cultured with lymph. The insulin content in lymph was statistically higher in lymph ( P < 0.001) than in plasma.CONCLUSIONS: The adipogenesis of ASCs was promoted under the lymph-cultured environment with statistically increased adipogenesis genes of peroxisome proliferator-activated receptor, CAAT/enhancer-binding protein α, fatty-acid binding protein, and lipoprotein lipase. The excess lymph accumulated in the lymphedematous extremity contained a greater insulin/insulin-like growth factor-2. These adipogenic factors promoted the expression of early adipogenesis genes and led ASCs to undergo adipogenesis and differentiated into adipocytes.CLINICAL RELEVANCE STATEMENT: The accumulation of adipose tissue in the lymphedema region was contributed from the content of excess lymph.

KW - Humans

KW - Adipogenesis/physiology

KW - Lipoprotein Lipase/metabolism

KW - Peroxisome Proliferator-Activated Receptors/metabolism

KW - Adipocytes/physiology

KW - Adipose Tissue

KW - Cell Differentiation/genetics

KW - Stem Cells/physiology

KW - Lymphedema

KW - Insulins/metabolism

KW - Cells, Cultured

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U2 - 10.1097/PRS.0000000000010082

DO - 10.1097/PRS.0000000000010082

M3 - 文章

C2 - 36534068

AN - SCOPUS:85158880738

SN - 0032-1052

VL - 151

SP - 1005

EP - 1015

JO - Plastic and Reconstructive Surgery

JF - Plastic and Reconstructive Surgery

IS - 5

ER -

The Impacts of Lymph on the Adipogenesis of Adipose-Derived Stem Cells

Abstract

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