The PHD domain of the sea urchin RAG2 homolog, SpRAG2L, recognizes dimethylated lysine 4 in histone H3 tails

David R. Wilson, Darrell D. Norton, Sebastian D. Fugmann*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

18 Scopus citations

Abstract

V(D)J recombination is a somatic gene rearrangement process that assembles antigen receptor genes from individual segments during lymphocyte development. The access of the RAG1/RAG2 recombinase to these gene segments is regulated at the level of chromatin modifications, in particular histone tail modifications. Trimethylation of lysine 4 in histone H3 (H3K4me3) correlates with actively recombining gene elements, and this mark is recognized and interpreted by the plant homeodomain (PHD) of RAG2. Here we report that the PHD domain of the only known invertebrate homolog of RAG2, the SpRAG2L protein of the purple sea urchin (Strongylocentrotus purpuratus) also binds to methylated histones, but with a unique preference for H3K4me2. While the cognate substrate for the sea urchin RAG1L/RAG2L complex remains elusive, the affinity for histone tails and the nuclear localization of ectopically expressed SpRAG2L strongly support the model that this enzyme complex exerts its activity on DNA in the context of chromatin.

Original languageEnglish
Pages (from-to)1221-1230
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume32
Issue number10
DOIs
StatePublished - 2008
Externally publishedYes

Keywords

  • Chromatin
  • H3K4 methylation
  • PHD domain
  • RAG2
  • SpRAG1L
  • SpRAG2L
  • Strongylocentrotus purpuratus
  • V(D)J recombination

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