The protective mechanism of magnolol, a Chinese herb drug, against warm ischemia-reperfusion injury of rat liver

Bruno Jawan, Shigeru Goto, Tair Long Pan, Chia Yun Lai, Hsiang Ning Luk, Hock Liew Eng, Yu Chun Lin, Yaw Sen Chen, Kok Mao Lan, Sao Wei Hsieh, Chih Chi Wang, Yu Fan Cheng, Chao Long Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

28 Scopus citations


Background. Cell apoptosis following warm ischemia-reperfusion injury is a major concern in clinical issues such as organ transplantation, trauma, and cardiogenic shock. The purpose of this study was to evaluate the possible role of magnolol, a Chinese herb drug, in apoptotic injury and the kinetic expression of apoptotic-related genes in rat livers subjected to warm ischemia-reperfusion (WI/R). Materials and methods. Three weeks prior to the experiment 10 rats underwent a portosystemic shunt operation according to Bengmerk's method. The rats were divided into three groups. Group 1 (GI) was the control group, Group 2 (GII) and Group 3 (GIII) the magnolol-treated groups. GI and GII were subjected to 2 h and GIII to 3 h of WI/R by clamping the portal vein and hepatic artery under ether anesthesia. Results. Results show that all the control rats died after 2 h WI/R. Apoptotic cells were detected under microscopy as well as by DNA assay. Magnolol-treated groups tolerated warm ischemia-reperfusion for 2 h and significantly less apoptotic cells were observed (198 ± 22 vs 42.6 ± 28). But magnolol-treated rats could not tolerate 3 h warm ischemia-reperfusion. RT-PCR of liver tissue shows that there is an upregulated expression of the anti-apoptotic Bcl-xL gene and suppression of the Bcl-xS gene in GII. Conclusion. Magnolol has an anti-apoptotic effect and protects the liver against WI/R for 2 h but not for 3 h through upregulation of the anti-apoptotic Bcl-XL gene and suppression of the Bcl-xS gene.

Original languageEnglish
Pages (from-to)378-382
Number of pages5
JournalJournal of Surgical Research
Issue number2
StatePublished - 04 2003


  • Animal
  • Apoptotic-related gene
  • Bcl-2
  • Bcl-xL
  • Bcl-xS
  • Liver, injury: warm ischemia-reperfusion
  • Organ
  • Rat


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