The Relationship of Pronuclear Stage Morphology and Chromosome Status at Cleavage Stage

Chun Kai Chen*, Guan Yi Shen, Shang Gwo Horng, Chia Woei Wang, Hong Yuan Huang, Hsin Shin Wang, Yung Kuei Soong

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

17 Scopus citations


Purpose: Infertile couples undergoing in vitro fertilization-embryo transfer (IVF-ET) program were included to study the relationship of pronuclear stage morphology and chromosome status at cleavage stage. Methods: Eighteen to twenty-one hours after fertilization, zygotes were checked for pronuclear morphology with modified Scott Z-score system. After embryo transfer on day 3, arrested or non-transferred 2 PN embryos were spread for fluorescence in situ hybridization (FISH) staining of probes to chromosomes 18, X and Y. Results: Ninety-eight embryos were successfully fixed and stained. The chromosome status were recorded in each 2 PN score group: 7 (54%) of 13 embryos in Z2 group, 14 (35%) of 40 in Z3 group and 10 (36%) of 28 in Z4 group being normal diploid. Z1 group has 12 (71%) of 17 embryos being normal diploid, which is significantly more than Z3 (p = 0.020) and Z4 group (p = 0.033). Conclusion: Our results demonstrated a high probability to get normal diploid embryos if good morphology at pronuclear stage was used as selection criteria, especially for Z1 score embryos.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalJournal of Assisted Reproduction and Genetics
Issue number10
StatePublished - 10 2003
Externally publishedYes


  • Chromosome status
  • Fluorescence in situ hybridization (FISH)
  • Pronuclear morphology
  • Zygote scoring


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