The reliability and predictive ability of a biomarker of oxidative DNA damage on functional outcomes after stroke rehabilitation

Yu Wei Hsieh, Keh Chung Lin, Mallikarjuna Korivi, Tsong Hai Lee, Ching Yi Wu*, Kuen Yuh Wu

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

26 Scopus citations

Abstract

We evaluated the reliability of 8-hydroxy-2'-deoxyguanosine (8-OHdG), and determined its ability to predict functional outcomes in stroke survivors. The rehabilitation effect on 8-OHdG and functional outcomes were also assessed. Sixty-one stroke patients received a 4-week rehabilitation. Urinary 8-OHdG levels were determined by liquid chromatography-tandem mass spectrometry. The test-retest reliability of 8-OHdG was good (interclass correlation coefficient = 0.76). Upper-limb motor function and muscle power determined by the Fugl-Meyer Assessment (FMA) and Medical Research Council (MRC) scales before rehabilitation showed significant negative correlation with 8-OHdG (r = -0.38, r = -0.30; p < 0.05). After rehabilitation, we found a fair and significantcorrelation between 8-OHdG and FMA (r = -0.34) and 8-OHdG and pain (r = 0.26, p < 0.05). Baseline 8-OHdG was significantly correlated with post-treatment FMA, MRC, and pain scores (r = -0.34, -0.31, and 0.25; p < 0.05), indicating its ability to predict functional outcomes. 8-OHdG levels were significantly decreased, and functional outcomes were improved after rehabilitation. The exploratory study findings conclude that 8-OHdG is a reliable and promising biomarker of oxidative stress and could be a valid predictor of functional outcomes in patients. Monitoring of behavioral indicators along with biomarkers may have crucial benefits in translational stroke research.

Original languageEnglish
Pages (from-to)6504-6516
Number of pages13
JournalInternational Journal of Molecular Sciences
Volume15
Issue number4
DOIs
StatePublished - 16 04 2014

Keywords

  • 8-OHdG
  • Biomarker
  • Clinical evaluation
  • Oxidative stress
  • Rehabilitation
  • Stroke

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