The role of dopamine transporter imaging agent [99mTc]TRODAT-1 in hemi-parkinsonism rat brain

Yen Kung Chen; Ren Shyan Liu; Wen Sheng Huang; Shiaw Pyng Wey; Gann Ting; Jiang Chuan Liu; Yeh You Shen; Fang Jung Wan

doi:10.1016/S0969-8051(01)00255-4

The role of dopamine transporter imaging agent [^99mTc]TRODAT-1 in hemi-parkinsonism rat brain

Yen Kung Chen, Ren Shyan Liu, Wen Sheng Huang, Shiaw Pyng Wey, Gann Ting, Jiang Chuan Liu, Yeh You Shen, Fang Jung Wan

Research output: Contribution to journal › Journal Article › peer-review

12 Scopus citations

Abstract

This study aims to investigate the relationship between the determination of dopamine level by high performance liquid chromatography (HPLC) with electrochemical detection (ECD) and the detection of dopamine transporter (DAT) counts using autoradiography with DAT image agent [^99mTc]TRODAT-1. For striatal lesions, pretreatment of 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle shows that autoradiogaphic labeling of striatum region is reduced to near-background level. Using HPLC with ECD, unilateral 6-OHDA treatment is associated with significant (p < 0.0002) reductions of dopamine levels. For the striatum of the 6-OHDA-lesioned side, dopamine content and DAT counts are reduced to 97% and 90%, respectively. Thus, our observation indicates a potential of using [^99mTc]TRODAT-1 for the evaluation of animal DAT.

Original language	English
Pages (from-to)	923-928
Number of pages	6
Journal	Nuclear Medicine and Biology
Volume	28
Issue number	8
DOIs	https://doi.org/10.1016/S0969-8051(01)00255-4
State	Published - 2001
Externally published	Yes

Keywords

6-OHDA
Dopamine
Dopamine transporter
[Tc]TRODAT-1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0969-8051(01)00255-4

Cite this

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title = "The role of dopamine transporter imaging agent [99mTc]TRODAT-1 in hemi-parkinsonism rat brain",

abstract = "This study aims to investigate the relationship between the determination of dopamine level by high performance liquid chromatography (HPLC) with electrochemical detection (ECD) and the detection of dopamine transporter (DAT) counts using autoradiography with DAT image agent [99mTc]TRODAT-1. For striatal lesions, pretreatment of 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle shows that autoradiogaphic labeling of striatum region is reduced to near-background level. Using HPLC with ECD, unilateral 6-OHDA treatment is associated with significant (p < 0.0002) reductions of dopamine levels. For the striatum of the 6-OHDA-lesioned side, dopamine content and DAT counts are reduced to 97% and 90%, respectively. Thus, our observation indicates a potential of using [99mTc]TRODAT-1 for the evaluation of animal DAT.",

keywords = "6-OHDA, Dopamine, Dopamine transporter, [Tc]TRODAT-1",

author = "Chen, {Yen Kung} and Liu, {Ren Shyan} and Huang, {Wen Sheng} and Wey, {Shiaw Pyng} and Gann Ting and Liu, {Jiang Chuan} and Shen, {Yeh You} and Wan, {Fang Jung}",

year = "2001",

doi = "10.1016/S0969-8051(01)00255-4",

language = "英语",

volume = "28",

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journal = "Nuclear Medicine and Biology",

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T1 - The role of dopamine transporter imaging agent [99mTc]TRODAT-1 in hemi-parkinsonism rat brain

AU - Chen, Yen Kung

AU - Liu, Ren Shyan

AU - Huang, Wen Sheng

AU - Wey, Shiaw Pyng

AU - Ting, Gann

AU - Liu, Jiang Chuan

AU - Shen, Yeh You

AU - Wan, Fang Jung

PY - 2001

Y1 - 2001

N2 - This study aims to investigate the relationship between the determination of dopamine level by high performance liquid chromatography (HPLC) with electrochemical detection (ECD) and the detection of dopamine transporter (DAT) counts using autoradiography with DAT image agent [99mTc]TRODAT-1. For striatal lesions, pretreatment of 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle shows that autoradiogaphic labeling of striatum region is reduced to near-background level. Using HPLC with ECD, unilateral 6-OHDA treatment is associated with significant (p < 0.0002) reductions of dopamine levels. For the striatum of the 6-OHDA-lesioned side, dopamine content and DAT counts are reduced to 97% and 90%, respectively. Thus, our observation indicates a potential of using [99mTc]TRODAT-1 for the evaluation of animal DAT.

AB - This study aims to investigate the relationship between the determination of dopamine level by high performance liquid chromatography (HPLC) with electrochemical detection (ECD) and the detection of dopamine transporter (DAT) counts using autoradiography with DAT image agent [99mTc]TRODAT-1. For striatal lesions, pretreatment of 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle shows that autoradiogaphic labeling of striatum region is reduced to near-background level. Using HPLC with ECD, unilateral 6-OHDA treatment is associated with significant (p < 0.0002) reductions of dopamine levels. For the striatum of the 6-OHDA-lesioned side, dopamine content and DAT counts are reduced to 97% and 90%, respectively. Thus, our observation indicates a potential of using [99mTc]TRODAT-1 for the evaluation of animal DAT.

KW - 6-OHDA

KW - Dopamine

KW - Dopamine transporter

KW - [Tc]TRODAT-1

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