The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli

Lih Hwa S. Hwang; Bing Fung Chen; Huei Ming Lin; Lai Ling Soong; Yuh Shan Jou; Wen Chang Chang; Shih Tung Liu

doi:10.1007/BF01024427

The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli

Lih Hwa S. Hwang^*, Bing Fung Chen, Huei Ming Lin, Lai Ling Soong, Yuh Shan Jou, Wen Chang Chang, Shih Tung Liu

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

5 Scopus citations

Abstract

Two types of expression vectors containing the P_tacor P_Lpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT₁T₂) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT₁T₂ terminator was essential for the efficient expression of pGH in the P_tac-driven vectors, but reduced the synthesis of pGH in the P_L-vectors. A leaky repression was observed in the JM103 cells containing the P_tac-plasmids.

Original language	English
Pages (from-to)	335-340
Number of pages	6
Journal	Biotechnology Letters
Volume	12
Issue number	5
DOIs	https://doi.org/10.1007/BF01024427
State	Published - 05 1990
Externally published	Yes

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title = "The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli",

abstract = "Two types of expression vectors containing the Ptacor PLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression of pGH in the Ptac-driven vectors, but reduced the synthesis of pGH in the PL-vectors. A leaky repression was observed in the JM103 cells containing the Ptac-plasmids.",

author = "Hwang, {Lih Hwa S.} and Chen, {Bing Fung} and Lin, {Huei Ming} and Soong, {Lai Ling} and Jou, {Yuh Shan} and Chang, {Wen Chang} and Liu, {Shih Tung}",

year = "1990",

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volume = "12",

pages = "335--340",

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TY - JOUR

T1 - The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli

AU - Hwang, Lih Hwa S.

AU - Chen, Bing Fung

AU - Lin, Huei Ming

AU - Soong, Lai Ling

AU - Jou, Yuh Shan

AU - Chang, Wen Chang

AU - Liu, Shih Tung

PY - 1990/5

Y1 - 1990/5

N2 - Two types of expression vectors containing the Ptacor PLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression of pGH in the Ptac-driven vectors, but reduced the synthesis of pGH in the PL-vectors. A leaky repression was observed in the JM103 cells containing the Ptac-plasmids.

AB - Two types of expression vectors containing the Ptacor PLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression of pGH in the Ptac-driven vectors, but reduced the synthesis of pGH in the PL-vectors. A leaky repression was observed in the JM103 cells containing the Ptac-plasmids.

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DO - 10.1007/BF01024427

M3 - 文章

AN - SCOPUS:0025168953

SN - 0141-5492

VL - 12

SP - 335

EP - 340

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 5

ER -

The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli

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