The structure of the mouse lymphocyte pore-forming protein perforin

Byoung S. Kwon; Mark Wakulchik; Chau Ching Liu; Pedro M. Persechini; Joseph A. Trapani; Asifa K. Haq; Yeong Kim; John Ding E. Young

doi:10.1016/S0006-291X(89)80168-8

The structure of the mouse lymphocyte pore-forming protein perforin

Byoung S. Kwon^*
, Mark Wakulchik
, Chau Ching Liu
, Pedro M. Persechini
, Joseph A. Trapani
, Asifa K. Haq
, Yeong Kim
, John Ding E. Young

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

61 Scopus citations

Abstract

Purified murine lymphocyte pore-forming protein (PFP or perforin) was partially sequenced. Oligonucleotides synthesized on the basis of this sequence information were used to screen a murine cytotoxic T lymphocyte (CTL) cDNA library. Seven clones were obtained, two of which were sequenced, providing full-length sequence information on PFP. Murine PFP (534 a.a.) is 68% identical to human PFP. Hydropathic analysis revealed a predominantly hydrophilic protein with some hydrophobic domains, including a region (a.a. 191-251) that could contain putative membrane-spanning domains. PFP is approx. 20% identical to human C7, C8 and C9 within a region encompassing 270 a.a., confirming previous immunological cross-reactivity studies. Northern blot analysis showed that expression of PFP but not of a serine esterase transcript is enhanced in a CTL line by antigen receptor-stimulation. Southern blot analysis of mouse genomic DNA indicated that PFP is encoded as a single-copy gene with the coding region contained within 10 kilobases of genomic DNA.

Original language	English
Pages (from-to)	1-10
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	158
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(89)80168-8
State	Published - 16 01 1989
Externally published	Yes

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title = "The structure of the mouse lymphocyte pore-forming protein perforin",

abstract = "Purified murine lymphocyte pore-forming protein (PFP or perforin) was partially sequenced. Oligonucleotides synthesized on the basis of this sequence information were used to screen a murine cytotoxic T lymphocyte (CTL) cDNA library. Seven clones were obtained, two of which were sequenced, providing full-length sequence information on PFP. Murine PFP (534 a.a.) is 68\% identical to human PFP. Hydropathic analysis revealed a predominantly hydrophilic protein with some hydrophobic domains, including a region (a.a. 191-251) that could contain putative membrane-spanning domains. PFP is approx. 20\% identical to human C7, C8 and C9 within a region encompassing 270 a.a., confirming previous immunological cross-reactivity studies. Northern blot analysis showed that expression of PFP but not of a serine esterase transcript is enhanced in a CTL line by antigen receptor-stimulation. Southern blot analysis of mouse genomic DNA indicated that PFP is encoded as a single-copy gene with the coding region contained within 10 kilobases of genomic DNA.",

author = "Kwon, \{Byoung S.\} and Mark Wakulchik and Liu, \{Chau Ching\} and Persechini, \{Pedro M.\} and Trapani, \{Joseph A.\} and Haq, \{Asifa K.\} and Yeong Kim and Young, \{John Ding E.\}",

year = "1989",

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doi = "10.1016/S0006-291X(89)80168-8",

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T1 - The structure of the mouse lymphocyte pore-forming protein perforin

AU - Kwon, Byoung S.

AU - Wakulchik, Mark

AU - Liu, Chau Ching

AU - Persechini, Pedro M.

AU - Trapani, Joseph A.

AU - Haq, Asifa K.

AU - Kim, Yeong

AU - Young, John Ding E.

PY - 1989/1/16

Y1 - 1989/1/16

N2 - Purified murine lymphocyte pore-forming protein (PFP or perforin) was partially sequenced. Oligonucleotides synthesized on the basis of this sequence information were used to screen a murine cytotoxic T lymphocyte (CTL) cDNA library. Seven clones were obtained, two of which were sequenced, providing full-length sequence information on PFP. Murine PFP (534 a.a.) is 68% identical to human PFP. Hydropathic analysis revealed a predominantly hydrophilic protein with some hydrophobic domains, including a region (a.a. 191-251) that could contain putative membrane-spanning domains. PFP is approx. 20% identical to human C7, C8 and C9 within a region encompassing 270 a.a., confirming previous immunological cross-reactivity studies. Northern blot analysis showed that expression of PFP but not of a serine esterase transcript is enhanced in a CTL line by antigen receptor-stimulation. Southern blot analysis of mouse genomic DNA indicated that PFP is encoded as a single-copy gene with the coding region contained within 10 kilobases of genomic DNA.

AB - Purified murine lymphocyte pore-forming protein (PFP or perforin) was partially sequenced. Oligonucleotides synthesized on the basis of this sequence information were used to screen a murine cytotoxic T lymphocyte (CTL) cDNA library. Seven clones were obtained, two of which were sequenced, providing full-length sequence information on PFP. Murine PFP (534 a.a.) is 68% identical to human PFP. Hydropathic analysis revealed a predominantly hydrophilic protein with some hydrophobic domains, including a region (a.a. 191-251) that could contain putative membrane-spanning domains. PFP is approx. 20% identical to human C7, C8 and C9 within a region encompassing 270 a.a., confirming previous immunological cross-reactivity studies. Northern blot analysis showed that expression of PFP but not of a serine esterase transcript is enhanced in a CTL line by antigen receptor-stimulation. Southern blot analysis of mouse genomic DNA indicated that PFP is encoded as a single-copy gene with the coding region contained within 10 kilobases of genomic DNA.

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DO - 10.1016/S0006-291X(89)80168-8

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AN - SCOPUS:0024557362

SN - 0006-291X

VL - 158

SP - 1

EP - 10

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

The structure of the mouse lymphocyte pore-forming protein perforin

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