Thermally-induced order-order transition of DNA-cationic surfactant complexes

Wei Long Hsu; Yen Cheng Li; Hsin Lung Chen; Willisa Liou; U. Ser Jeng; Hsien Kuang Lin; Wen Liang Liu; Chain Shu Hsu

doi:10.1021/la060862z

Thermally-induced order-order transition of DNA-cationic surfactant complexes

Wei Long Hsu
, Yen Cheng Li
, Hsin Lung Chen^*
, Willisa Liou
, U. Ser Jeng
, Hsien Kuang Lin
, Wen Liang Liu
, Chain Shu Hsu

^*Corresponding author for this work

Department of Anatomy

Research output: Contribution to journal › Journal Article › peer-review

20 Scopus citations

Abstract

Polyanionic DNA interacts with cationic amphiphiles to form electrostatic complexes exhibiting rich self-assembled structures. This type of complex has been considered as a nonviral carrier in gene therapy and as a template for nanostructure construction. Here we report a thermally-induced phase transition of the complexes of DNA with the mixtures of a cationic surfactant, dodecyltrimethyl bromide (DTAB), and a neutral lipid, dioleoylphosphatidylethanolamine (DOPE), in fully hydrated state. An order-order transition between a multilamellar (L_α^c phase and an inverted hexagonal (H_II^c) phase was found to occur with the transition temperature adjustable by the DTAB-to-DNA base pair molar ratio (x) and DOPE-to-DTAB molar ratio (m). The stability of the L _α^c phase was enhanced at lower m and x, as the L_α^c-to-H_II^c transition temperature increased with the decreases of these two parameters. The suppression of L_α^c-to-H_II^c transition at lower x was attributed to the lower entropie gain from the counterion release due to the oresence of uncomolexed DNA in the bulk solution.

Original language	English
Pages (from-to)	7521-7527
Number of pages	7
Journal	Langmuir
Volume	22
Issue number	18
DOIs	https://doi.org/10.1021/la060862z
State	Published - 29 08 2006

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title = "Thermally-induced order-order transition of DNA-cationic surfactant complexes",

abstract = "Polyanionic DNA interacts with cationic amphiphiles to form electrostatic complexes exhibiting rich self-assembled structures. This type of complex has been considered as a nonviral carrier in gene therapy and as a template for nanostructure construction. Here we report a thermally-induced phase transition of the complexes of DNA with the mixtures of a cationic surfactant, dodecyltrimethyl bromide (DTAB), and a neutral lipid, dioleoylphosphatidylethanolamine (DOPE), in fully hydrated state. An order-order transition between a multilamellar (Lαc phase and an inverted hexagonal (HIIc) phase was found to occur with the transition temperature adjustable by the DTAB-to-DNA base pair molar ratio (x) and DOPE-to-DTAB molar ratio (m). The stability of the L αc phase was enhanced at lower m and x, as the Lαc-to-HIIc transition temperature increased with the decreases of these two parameters. The suppression of Lαc-to-HIIc transition at lower x was attributed to the lower entropie gain from the counterion release due to the oresence of uncomolexed DNA in the bulk solution.",

author = "Hsu, \{Wei Long\} and Li, \{Yen Cheng\} and Chen, \{Hsin Lung\} and Willisa Liou and Jeng, \{U. Ser\} and Lin, \{Hsien Kuang\} and Liu, \{Wen Liang\} and Hsu, \{Chain Shu\}",

year = "2006",

month = aug,

day = "29",

doi = "10.1021/la060862z",

language = "英语",

volume = "22",

pages = "7521--7527",

journal = "Langmuir",

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publisher = "American Chemical Society",

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T1 - Thermally-induced order-order transition of DNA-cationic surfactant complexes

AU - Hsu, Wei Long

AU - Li, Yen Cheng

AU - Chen, Hsin Lung

AU - Liou, Willisa

AU - Jeng, U. Ser

AU - Lin, Hsien Kuang

AU - Liu, Wen Liang

AU - Hsu, Chain Shu

PY - 2006/8/29

Y1 - 2006/8/29

N2 - Polyanionic DNA interacts with cationic amphiphiles to form electrostatic complexes exhibiting rich self-assembled structures. This type of complex has been considered as a nonviral carrier in gene therapy and as a template for nanostructure construction. Here we report a thermally-induced phase transition of the complexes of DNA with the mixtures of a cationic surfactant, dodecyltrimethyl bromide (DTAB), and a neutral lipid, dioleoylphosphatidylethanolamine (DOPE), in fully hydrated state. An order-order transition between a multilamellar (Lαc phase and an inverted hexagonal (HIIc) phase was found to occur with the transition temperature adjustable by the DTAB-to-DNA base pair molar ratio (x) and DOPE-to-DTAB molar ratio (m). The stability of the L αc phase was enhanced at lower m and x, as the Lαc-to-HIIc transition temperature increased with the decreases of these two parameters. The suppression of Lαc-to-HIIc transition at lower x was attributed to the lower entropie gain from the counterion release due to the oresence of uncomolexed DNA in the bulk solution.

AB - Polyanionic DNA interacts with cationic amphiphiles to form electrostatic complexes exhibiting rich self-assembled structures. This type of complex has been considered as a nonviral carrier in gene therapy and as a template for nanostructure construction. Here we report a thermally-induced phase transition of the complexes of DNA with the mixtures of a cationic surfactant, dodecyltrimethyl bromide (DTAB), and a neutral lipid, dioleoylphosphatidylethanolamine (DOPE), in fully hydrated state. An order-order transition between a multilamellar (Lαc phase and an inverted hexagonal (HIIc) phase was found to occur with the transition temperature adjustable by the DTAB-to-DNA base pair molar ratio (x) and DOPE-to-DTAB molar ratio (m). The stability of the L αc phase was enhanced at lower m and x, as the Lαc-to-HIIc transition temperature increased with the decreases of these two parameters. The suppression of Lαc-to-HIIc transition at lower x was attributed to the lower entropie gain from the counterion release due to the oresence of uncomolexed DNA in the bulk solution.

UR - https://www.scopus.com/pages/publications/33748560127

U2 - 10.1021/la060862z

DO - 10.1021/la060862z

M3 - 文章

C2 - 16922529

AN - SCOPUS:33748560127

SN - 0743-7463

VL - 22

SP - 7521

EP - 7527

JO - Langmuir

JF - Langmuir

IS - 18

ER -

Thermally-induced order-order transition of DNA-cationic surfactant complexes

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