Third and second harmonic generation imaging of human articular cartilage

Ming Rung Tsai; Chih Hwa Chen; Chi Kuang Sun

doi:10.1117/12.809968

Third and second harmonic generation imaging of human articular cartilage

Ming Rung Tsai^*
, Chih Hwa Chen
, Chi Kuang Sun

^*Corresponding author for this work

National Taiwan University
Chang Gung Memorial Hospital
Academia Sinica - Research Center for Applied Science

Research output: Contribution to journal › Conference article › peer-review

8 Scopus citations

Abstract

Cartilage matrix is damaged in diseased states such as osteoarthritis while adult articular cartilage does not have the capacity to repair structural damage. A least invasive mean to diagnose these diseased states of human articular cartilage with a high spatial resolution is thus highly desired. In this paper we present our harmonic generation microscopic studies on the human articular cartilage samples. Without any staining third and second harmonic generation can provide strong contrast in chondrocytes and collagen matrix respectively. Our study indicates the high capability of harmonic generation microscopy for future articular cartilage disease diagnosis.

Original language	English
Article number	71831V
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7183
DOIs	https://doi.org/10.1117/12.809968
State	Published - 2009
Externally published	Yes
Event	Multiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States Duration: 25 01 2009 → 27 01 2009

Keywords

3D microscopy
Articular cartilage
Chondrocyte
Harmonic generation microscopy

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10.1117/12.809968

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title = "Third and second harmonic generation imaging of human articular cartilage",

abstract = "Cartilage matrix is damaged in diseased states such as osteoarthritis while adult articular cartilage does not have the capacity to repair structural damage. A least invasive mean to diagnose these diseased states of human articular cartilage with a high spatial resolution is thus highly desired. In this paper we present our harmonic generation microscopic studies on the human articular cartilage samples. Without any staining third and second harmonic generation can provide strong contrast in chondrocytes and collagen matrix respectively. Our study indicates the high capability of harmonic generation microscopy for future articular cartilage disease diagnosis.",

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T1 - Third and second harmonic generation imaging of human articular cartilage

AU - Tsai, Ming Rung

AU - Chen, Chih Hwa

AU - Sun, Chi Kuang

PY - 2009

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N2 - Cartilage matrix is damaged in diseased states such as osteoarthritis while adult articular cartilage does not have the capacity to repair structural damage. A least invasive mean to diagnose these diseased states of human articular cartilage with a high spatial resolution is thus highly desired. In this paper we present our harmonic generation microscopic studies on the human articular cartilage samples. Without any staining third and second harmonic generation can provide strong contrast in chondrocytes and collagen matrix respectively. Our study indicates the high capability of harmonic generation microscopy for future articular cartilage disease diagnosis.

AB - Cartilage matrix is damaged in diseased states such as osteoarthritis while adult articular cartilage does not have the capacity to repair structural damage. A least invasive mean to diagnose these diseased states of human articular cartilage with a high spatial resolution is thus highly desired. In this paper we present our harmonic generation microscopic studies on the human articular cartilage samples. Without any staining third and second harmonic generation can provide strong contrast in chondrocytes and collagen matrix respectively. Our study indicates the high capability of harmonic generation microscopy for future articular cartilage disease diagnosis.

KW - 3D microscopy

KW - Articular cartilage

KW - Chondrocyte

KW - Harmonic generation microscopy

UR - https://www.scopus.com/pages/publications/65949101230

U2 - 10.1117/12.809968

DO - 10.1117/12.809968

M3 - 会议文章

AN - SCOPUS:65949101230

SN - 1605-7422

VL - 7183

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 71831V

T2 - Multiphoton Microscopy in the Biomedical Sciences IX

Y2 - 25 January 2009 through 27 January 2009

ER -

Third and second harmonic generation imaging of human articular cartilage

Abstract

Keywords

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