TPH1 and 5-HTTLPR genes specifically interact in opiate dependence but not in alcohol dependence

Tzu Yun Wang; Sheng Yu Lee; Yi Lun Chung; Shiou Lan Chen; Chia Ling Li; Yun Hsuan Chang; Liang Jen Wang; Po See Chen; Shih Heng Chen; Chun Hsien Chu; San Yuan Huang; Nian Sheng Tzeng; Tsai Hsin Hsieh; I. Hui Lee; Kao Chin Chen; Yen Kuang Yang; Jau Shyong Hong; Ru Band Lu

doi:10.1159/000444676

TPH1 and 5-HTTLPR genes specifically interact in opiate dependence but not in alcohol dependence

Tzu Yun Wang, Sheng Yu Lee, Yi Lun Chung, Shiou Lan Chen, Chia Ling Li, Yun Hsuan Chang, Liang Jen Wang, Po See Chen, Shih Heng Chen, Chun Hsien Chu, San Yuan Huang, Nian Sheng Tzeng, Tsai Hsin Hsieh, I. Hui Lee, Kao Chin Chen, Yen Kuang Yang, Jau Shyong Hong, Ru Band Lu^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

Background: Different drug dependencies may have unique genetic vulnerabilities. Changes in serotonin availability and function have been linked to addiction. We investigated whether 2 serotonergic polymorphisms, TPH1 A218C (rs1800532) and 5-HTT-linked promoter region (5-HTTLPR) (rs25531), are differently associated with alcohol or opiate dependence. Methods: Alcohol-dependent patients (n = 292), opiate-dependent patients (n = 309), and healthy controls (n = 301) were recruited from the Han Chinese population in Taiwan. Genotypes of TPH1 A218C and 5-HTTLPR polymorphisms were analyzed using a polymerase chain reaction with restriction fragment length polymorphism. Results: The genotype frequencies of the TPH1 A218C polymorphisms were not significantly different in the 3 groups. The genotype frequencies of the 5-HTTLPR S+ (S/S, S/L_G, L_G/L_G) polymorphisms were significantly higher in opiate-dependent patients (χ² = 8.77, p = 0.01), but not after controlling for the covariates of age, gender, and interaction effect in logistic regression analysis. Moreover, there was a significant interaction between the TPH1 A218C A/C and 5-HTTLPR S+ gene polymorphisms in opiate-dependent (OR 2.72, p = 0.01), but not in alcohol-dependent patients. Conclusions: Our data suggested that there may be a differential genetic vulnerability in serotonergic genes for alcohol and opiate addiction. However, replications of our findings are still needed.

Original language	English
Pages (from-to)	201-209
Number of pages	9
Journal	European Addiction Research
Volume	22
Issue number	4
DOIs	https://doi.org/10.1159/000444676
State	Published - 01 06 2016

Bibliographical note

Publisher Copyright:
© 2016 S. Karger AG, Basel.

Keywords

5-HTT -linked promoter region
Alcohol dependence
Gene-to-gene interaction
Opiate dependence
TPH1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1159/000444676

Cite this

Wang, T. Y., Lee, S. Y., Chung, Y. L., Chen, S. L., Li, C. L., Chang, Y. H., Wang, L. J., Chen, P. S., Chen, S. H., Chu, C. H., Huang, S. Y., Tzeng, N. S., Hsieh, T. H., Lee, I. H., Chen, K. C., Yang, Y. K., Hong, J. S., & Lu, R. B. (2016). TPH1 and 5-HTTLPR genes specifically interact in opiate dependence but not in alcohol dependence. European Addiction Research, 22(4), 201-209. https://doi.org/10.1159/000444676

@article{4148c6e33272441185fdaa923437e089,

title = "TPH1 and 5-HTTLPR genes specifically interact in opiate dependence but not in alcohol dependence",

abstract = "Background: Different drug dependencies may have unique genetic vulnerabilities. Changes in serotonin availability and function have been linked to addiction. We investigated whether 2 serotonergic polymorphisms, TPH1 A218C (rs1800532) and 5-HTT-linked promoter region (5-HTTLPR) (rs25531), are differently associated with alcohol or opiate dependence. Methods: Alcohol-dependent patients (n = 292), opiate-dependent patients (n = 309), and healthy controls (n = 301) were recruited from the Han Chinese population in Taiwan. Genotypes of TPH1 A218C and 5-HTTLPR polymorphisms were analyzed using a polymerase chain reaction with restriction fragment length polymorphism. Results: The genotype frequencies of the TPH1 A218C polymorphisms were not significantly different in the 3 groups. The genotype frequencies of the 5-HTTLPR S+ (S/S, S/LG, LG/LG) polymorphisms were significantly higher in opiate-dependent patients (χ2 = 8.77, p = 0.01), but not after controlling for the covariates of age, gender, and interaction effect in logistic regression analysis. Moreover, there was a significant interaction between the TPH1 A218C A/C and 5-HTTLPR S+ gene polymorphisms in opiate-dependent (OR 2.72, p = 0.01), but not in alcohol-dependent patients. Conclusions: Our data suggested that there may be a differential genetic vulnerability in serotonergic genes for alcohol and opiate addiction. However, replications of our findings are still needed.",

keywords = "5-HTT -linked promoter region, Alcohol dependence, Gene-to-gene interaction, Opiate dependence, TPH1",

author = "Wang, {Tzu Yun} and Lee, {Sheng Yu} and Chung, {Yi Lun} and Chen, {Shiou Lan} and Li, {Chia Ling} and Chang, {Yun Hsuan} and Wang, {Liang Jen} and Chen, {Po See} and Chen, {Shih Heng} and Chu, {Chun Hsien} and Huang, {San Yuan} and Tzeng, {Nian Sheng} and Hsieh, {Tsai Hsin} and Lee, {I. Hui} and Chen, {Kao Chin} and Yang, {Yen Kuang} and Hong, {Jau Shyong} and Lu, {Ru Band}",

note = "Publisher Copyright: {\textcopyright} 2016 S. Karger AG, Basel.",

year = "2016",

month = jun,

day = "1",

doi = "10.1159/000444676",

language = "英语",

volume = "22",

pages = "201--209",

journal = "European Addiction Research",

issn = "1022-6877",

publisher = "S. Karger AG",

number = "4",

}

Wang, TY, Lee, SY, Chung, YL, Chen, SL, Li, CL, Chang, YH, Wang, LJ, Chen, PS, Chen, SH, Chu, CH, Huang, SY, Tzeng, NS, Hsieh, TH, Lee, IH, Chen, KC, Yang, YK, Hong, JS & Lu, RB 2016, 'TPH1 and 5-HTTLPR genes specifically interact in opiate dependence but not in alcohol dependence', European Addiction Research, vol. 22, no. 4, pp. 201-209. https://doi.org/10.1159/000444676

TY - JOUR

T1 - TPH1 and 5-HTTLPR genes specifically interact in opiate dependence but not in alcohol dependence

AU - Wang, Tzu Yun

AU - Lee, Sheng Yu

AU - Chung, Yi Lun

AU - Chen, Shiou Lan

AU - Li, Chia Ling

AU - Chang, Yun Hsuan

AU - Wang, Liang Jen

AU - Chen, Po See

AU - Chen, Shih Heng

AU - Chu, Chun Hsien

AU - Huang, San Yuan

AU - Tzeng, Nian Sheng

AU - Hsieh, Tsai Hsin

AU - Lee, I. Hui

AU - Chen, Kao Chin

AU - Yang, Yen Kuang

AU - Hong, Jau Shyong

AU - Lu, Ru Band

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Background: Different drug dependencies may have unique genetic vulnerabilities. Changes in serotonin availability and function have been linked to addiction. We investigated whether 2 serotonergic polymorphisms, TPH1 A218C (rs1800532) and 5-HTT-linked promoter region (5-HTTLPR) (rs25531), are differently associated with alcohol or opiate dependence. Methods: Alcohol-dependent patients (n = 292), opiate-dependent patients (n = 309), and healthy controls (n = 301) were recruited from the Han Chinese population in Taiwan. Genotypes of TPH1 A218C and 5-HTTLPR polymorphisms were analyzed using a polymerase chain reaction with restriction fragment length polymorphism. Results: The genotype frequencies of the TPH1 A218C polymorphisms were not significantly different in the 3 groups. The genotype frequencies of the 5-HTTLPR S+ (S/S, S/LG, LG/LG) polymorphisms were significantly higher in opiate-dependent patients (χ2 = 8.77, p = 0.01), but not after controlling for the covariates of age, gender, and interaction effect in logistic regression analysis. Moreover, there was a significant interaction between the TPH1 A218C A/C and 5-HTTLPR S+ gene polymorphisms in opiate-dependent (OR 2.72, p = 0.01), but not in alcohol-dependent patients. Conclusions: Our data suggested that there may be a differential genetic vulnerability in serotonergic genes for alcohol and opiate addiction. However, replications of our findings are still needed.

AB - Background: Different drug dependencies may have unique genetic vulnerabilities. Changes in serotonin availability and function have been linked to addiction. We investigated whether 2 serotonergic polymorphisms, TPH1 A218C (rs1800532) and 5-HTT-linked promoter region (5-HTTLPR) (rs25531), are differently associated with alcohol or opiate dependence. Methods: Alcohol-dependent patients (n = 292), opiate-dependent patients (n = 309), and healthy controls (n = 301) were recruited from the Han Chinese population in Taiwan. Genotypes of TPH1 A218C and 5-HTTLPR polymorphisms were analyzed using a polymerase chain reaction with restriction fragment length polymorphism. Results: The genotype frequencies of the TPH1 A218C polymorphisms were not significantly different in the 3 groups. The genotype frequencies of the 5-HTTLPR S+ (S/S, S/LG, LG/LG) polymorphisms were significantly higher in opiate-dependent patients (χ2 = 8.77, p = 0.01), but not after controlling for the covariates of age, gender, and interaction effect in logistic regression analysis. Moreover, there was a significant interaction between the TPH1 A218C A/C and 5-HTTLPR S+ gene polymorphisms in opiate-dependent (OR 2.72, p = 0.01), but not in alcohol-dependent patients. Conclusions: Our data suggested that there may be a differential genetic vulnerability in serotonergic genes for alcohol and opiate addiction. However, replications of our findings are still needed.

KW - 5-HTT -linked promoter region

KW - Alcohol dependence

KW - Gene-to-gene interaction

KW - Opiate dependence

KW - TPH1

UR - http://www.scopus.com/inward/record.url?scp=84964089220&partnerID=8YFLogxK

U2 - 10.1159/000444676

DO - 10.1159/000444676

M3 - 文章

C2 - 27045756

AN - SCOPUS:84964089220

SN - 1022-6877

VL - 22

SP - 201

EP - 209

JO - European Addiction Research

JF - European Addiction Research

IS - 4

ER -

TPH1 and 5-HTTLPR genes specifically interact in opiate dependence but not in alcohol dependence

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this