Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins

Tzu Hao Wang; Yun Shien Lee; Shiaw Min Hwang

doi:10.1007/978-1-60761-999-4_29

Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins

Tzu Hao Wang, Yun Shien Lee, Shiaw Min Hwang^*

^*Corresponding author for this work

Graduate Institute of Clinical Medical Sciences

Food Industry Research and Development Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

13 Scopus citations

Abstract

We have used Affymetrix oligonucleotide microarrays to analyze common transcriptomes and thereby learn about the core gene expression profile in human mesenchymal stem cells (MSC) from different tissues, including fetal amniotic fluid-derived MSC, term pregnancy amniotic membrane-derived MSC, term pregnancy umbilical cord blood-derived MSC, and adult bone marrow-derived MSC. The beauty of microarray analysis of gene expression (MAGE) is that it can be used to discover associating genes that were previously thought to be unrelated to a physiological or pathological event. However, interpreting complex biological processes from gene expression profiles often requires extensive knowledge mining in biomedical literature. In this chapter, we describe, step-by-step, how to use a commercially available biological database and software program, MetaCore (GeneGo Inc.), for functional network analysis.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	405-417
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-60761-999-4_29
State	Published - 2011

Publication series

Name	Methods in Molecular Biology
Volume	698
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

Keywords

Amniotic fluid
Amniotic membrane
Bone marrow
Cord blood
Functional network analysis
Mesenchymal stem cells
Metacore (GeneGo)

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10.1007/978-1-60761-999-4_29

Cite this

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abstract = "We have used Affymetrix oligonucleotide microarrays to analyze common transcriptomes and thereby learn about the core gene expression profile in human mesenchymal stem cells (MSC) from different tissues, including fetal amniotic fluid-derived MSC, term pregnancy amniotic membrane-derived MSC, term pregnancy umbilical cord blood-derived MSC, and adult bone marrow-derived MSC. The beauty of microarray analysis of gene expression (MAGE) is that it can be used to discover associating genes that were previously thought to be unrelated to a physiological or pathological event. However, interpreting complex biological processes from gene expression profiles often requires extensive knowledge mining in biomedical literature. In this chapter, we describe, step-by-step, how to use a commercially available biological database and software program, MetaCore (GeneGo Inc.), for functional network analysis.",

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Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins

Abstract

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Keywords

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