TY - JOUR
T1 - Transient global ischemia alters NMDA receptor expression in rat hippocampus
T2 - Correlation with decreased immunoreactive protein levels of the NR2A/2B subunits, and an altered NMDA receptor functionality
AU - Zhang,
AU - Hsu, Jee Ching
AU - Takagi, Norio
AU - Gurd, James W.
AU - Wallace, M. Christopher
AU - Eubanks, James H.
PY - 1997/11
Y1 - 1997/11
N2 - We investigated the gene expression levels, the immunoreactive protein prevalence, and the functional activity of N-methyl-D-aspartate (NMDA) receptor complexes at early times after severe global ischemia challenge in rats. The mRNA expression levels for the NR2A and NR2B subunits of NMDA receptors changed to different degrees within different subregions of the hippocampus after reperfusion with respect to sham-operated control. No significant change in expression was observed in the vulnerable CA1 subfield at or before 6 h after challenge for either receptor subunit, although changes in expression in other hippocampal subfields were observed. At 12 and 24 h after challenge, significant decreases in expression for both subunits were found in the vulnerable CA1 subfield, as well as in other hippocampal regions. At the protein level, a significant decrease in the amount of NR2A/NR2B immunoreactivity in the total hippocampus was observed at both 6 and 24 h after reperfusion compared with sham control. Electrophysiological assessment of single-channel NMDA receptor activity in the CA1 subfield indicates mat the main conductance state of NMDA receptor channels is maintained 6 h after challenge, although by 18-24 h after challenge, this main conductance state is rarely observed. The NMDA receptor component of the excitatory postsynaptic field potential was found to be significantly diminished from sham control 24 h after challenge, such that only ~10% of the sham response remained, but was not significantly altered from sham control at 8 h after challenge. These results indicate that decreases in the expression levels, the immunoreactive protein prevalence, and that alterations in the functionality of NMDA receptors occur in the hippocampus at early times after severe transient global ischemia.
AB - We investigated the gene expression levels, the immunoreactive protein prevalence, and the functional activity of N-methyl-D-aspartate (NMDA) receptor complexes at early times after severe global ischemia challenge in rats. The mRNA expression levels for the NR2A and NR2B subunits of NMDA receptors changed to different degrees within different subregions of the hippocampus after reperfusion with respect to sham-operated control. No significant change in expression was observed in the vulnerable CA1 subfield at or before 6 h after challenge for either receptor subunit, although changes in expression in other hippocampal subfields were observed. At 12 and 24 h after challenge, significant decreases in expression for both subunits were found in the vulnerable CA1 subfield, as well as in other hippocampal regions. At the protein level, a significant decrease in the amount of NR2A/NR2B immunoreactivity in the total hippocampus was observed at both 6 and 24 h after reperfusion compared with sham control. Electrophysiological assessment of single-channel NMDA receptor activity in the CA1 subfield indicates mat the main conductance state of NMDA receptor channels is maintained 6 h after challenge, although by 18-24 h after challenge, this main conductance state is rarely observed. The NMDA receptor component of the excitatory postsynaptic field potential was found to be significantly diminished from sham control 24 h after challenge, such that only ~10% of the sham response remained, but was not significantly altered from sham control at 8 h after challenge. These results indicate that decreases in the expression levels, the immunoreactive protein prevalence, and that alterations in the functionality of NMDA receptors occur in the hippocampus at early times after severe transient global ischemia.
KW - Cerebral ischemia
KW - Electrophysiology
KW - Gene expression
KW - NMDA receptor
UR - http://www.scopus.com/inward/record.url?scp=1842290343&partnerID=8YFLogxK
U2 - 10.1046/j.1471-4159.1997.69051983.x
DO - 10.1046/j.1471-4159.1997.69051983.x
M3 - 文章
C2 - 9349543
AN - SCOPUS:1842290343
SN - 0022-3042
VL - 69
SP - 1983
EP - 1994
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -