Abstract
Purpose: Partial bladder outlet obstruction causes a significant increase in tissue and systemic oxidative stress markers and tissue inflammatory cytokine levels. Myeloid-derived suppressor cells, γ, IL-10 and aldosterone are believed to be associated with oxidative stress and inflammation. We investigated alterations in plasma myeloid-derived suppressor cells, γ, IL-10 and aldosterone levels in partial bladder outlet obstruction and after its reversal.
Materials and Methods: Rats with surgically induced partial bladder outlet obstruction were divided into 4 groups of 3 each, including sham treated, 4-week obstruction, and 4 and 8-week obstruction with relief. Plasma levels of circulating myeloid-derived suppressor cells, γ, IL-10 and aldosterone were assessed by flow cytometry or enzyme-linked immunosorbent assay.
Results: The circulating myeloid-derived suppressor cell level was markedly increased in the obstruction group compared to the sham treated group and it returned to normal in the 4 and 8-week obstruction with relief groups. Plasma γ, IL-10 and aldosterone were similarly increased in the obstruction group and returned to normal in the 4 and 8-week obstruction with relief groups.
Conclusions: Levels of circulating myeloid-derived suppressor cells, γ, IL-10 and aldosterone were increased in rats with partial bladder outlet obstruction but returned to normal after reversal. This suggests that an increase in these parameters may be a good predictive indicator of patients at increased risk for urinary symptoms.
Original language | English |
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Pages (from-to) | 1569-1573 |
Number of pages | 5 |
Journal | Journal of Urology |
Volume | 192 |
Issue number | 5 |
DOIs | |
State | Published - 01 11 2014 |
Bibliographical note
Publisher Copyright:© 2014 American Urological Association Education and Research, Inc.
Keywords
- cell differentiation
- cytokines
- inflammation
- oxidative stress
- urinary bladder neck obstruction