Turnover of proglycogen and total glycogen in muscles of fasted rats infused with insulin during hepatic inflow occlusion

M. T. Huang*, C. F. Lee, R. F. Lin, R. J. Chcn

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

Abstract

Muscle glycogen turnover and plasma glucose utilization were studied in fasted rats infused i.v. with [B-3HJ-glucosc and insulin at rates of 0, 0.35, (1.77, 1.925 or 3.85 u.U/min during a 20min period of hepatic inflow occlusion. During the occlusion, plasma glucose and I act ate were found to decrease and increase, respectively, in all groups linearly. While the production of plasma lac late was not significantly affected, the utilisation of plasma glucose increased in the insulin-treatcd rats. The effect of insulin on plasma glucose plateaucd at a rate of infusion of 0.77 u. U/min (129%). Two separate methods were used to extract glycogen in hindlimb muscles freczc-clamped at the end of the infusion: small molecule glycogen (proglycogen) by precipitation with 10% trichloroacetic acid and total glycogen by precipitation with 30% cthanolic KOH at 100 C. While the levels of proglycogen and total glycogen were unchanged, labeled glucose incorporation into both fractions of glycogen increased significantly in the insulin-treated rats. With no apparent increase in glucose storage or glycolylic products, increased glucose utilization not accounted for by lac t ale production in the insulin-treated rats was considered to be oxidized. Based on Ihis assumption, the correlation between the increases in glucose utilization and label incorporation in muscle glycogen may indicate a link between insulin-stimulated glucose oxidation and glycogen turnover.

Original languageEnglish
Pages (from-to)A214
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

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