Two-frequency confocal laser scanning fluorescence microscope for specimen-induced spherical aberration reduction

Yung Chin Chung, Jheng Syong Wu, Chien Chou*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A two-frequency heterodyne confocal laser scanning fluorescence microscope (TF-CLSFM) is setup where a harmonic intensity modulated fluorescence signal is detected coherently. Due to the ability of reduction on refractive index mismatched spherical aberration in TF-CLSFM, it clearly shows that the performance of TF-CLSFM on axial response is better than conventional confocal laser scanning fluorescence microscope (CLSFM) according to the experimental results.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXI
PublisherSPIE
ISBN (Print)9780819498625
DOIs
StatePublished - 2014
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI - San Francisco, CA, United States
Duration: 03 02 201406 02 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8949
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI
Country/TerritoryUnited States
CitySan Francisco, CA
Period03/02/1406/02/14

Keywords

  • Confocal microscope
  • Optical heterodyne
  • Spherical aberration
  • Two-frequency laser

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