TY - JOUR
T1 - Two-Layer Method (UW Solution/Perfluorochemical Plus O2) for Lung Preservation in Rat Lung Transplantation
AU - Liu, C. C.
AU - Hsu, P. K.
AU - Huang, W. C.
AU - Huang, M. H.
AU - Hsu, H. S.
PY - 2007/12
Y1 - 2007/12
N2 - A new preservation method using perfluorochemicals (PFC) with oxygen administered continuously was developed for lung preservation and compared with traditional cold preservation methods for rat lung transplantation. Male Sprague-Dawley rats underwent orthotopic left lung transplantations of grafts preserved in lactiated Ringers solution (LR), University of Wisconsin solution (UW), Celsior solution, or a two-layer (PFC plus O2) solution for 6 hours. One hour after reperfusion, the right pulmonary artery and bronchus were clamped and 5 minutes later we recorded peak airway pressure and Pao2 level. The isograft was excised for measurement of myeloperoxidase activity, wet-to-dry ratio, and histologic examination to evaluate isograft function. The mean peak airway pressure was 29.80 ± 6.72 mm H2O in the LR group, 28.80 ± 5.76 mm H2O in the UW group, 33.60 ± 5.17 mm H2O in the Celsior group, and 32.40 ± 2.60 in the two-layer group. The mean Pao2 level was 99.78 ± 76.09 mm Hg in the LR group, 87.84 ± 33.58 mm Hg in the UW group, 104.50 ± 72.93 mm Hg in the Celsior group, and 62.08 ± 31.34 mm Hg in PFC and UW solution plus O2 group (two layers). The mean net myeloperoxidase activity OD level was 0.110 ± 0.104 in the LR group, 0.392 ± 0.328 in the UW group, 0.351 ± 0.620 in the Celsior group, and 0.532 ± 0.616 in the two-layer group. The mean wet-to-dry ratio was 7.47 ± 1.60 in the LR group, 6.56 ± 0.62 in the UW group, 7.54 ± 2.19 in the Celsior group, and 5.32 ± 2.20 in the two-layer group. The differences between groups in these parameters were not significant. Upon histologic examination, more inflammatory cell aggregates were seen in the two-layer group, less in the LR and the Celsior groups. The function of the lung graft after 6 hours of storage was not better using this two-layer method for preservation than traditional preservation methods in rat lung transplantation. Histologic examination revealed more inflammatory cell aggregates in the lung graft preserved using a two-layer method.
AB - A new preservation method using perfluorochemicals (PFC) with oxygen administered continuously was developed for lung preservation and compared with traditional cold preservation methods for rat lung transplantation. Male Sprague-Dawley rats underwent orthotopic left lung transplantations of grafts preserved in lactiated Ringers solution (LR), University of Wisconsin solution (UW), Celsior solution, or a two-layer (PFC plus O2) solution for 6 hours. One hour after reperfusion, the right pulmonary artery and bronchus were clamped and 5 minutes later we recorded peak airway pressure and Pao2 level. The isograft was excised for measurement of myeloperoxidase activity, wet-to-dry ratio, and histologic examination to evaluate isograft function. The mean peak airway pressure was 29.80 ± 6.72 mm H2O in the LR group, 28.80 ± 5.76 mm H2O in the UW group, 33.60 ± 5.17 mm H2O in the Celsior group, and 32.40 ± 2.60 in the two-layer group. The mean Pao2 level was 99.78 ± 76.09 mm Hg in the LR group, 87.84 ± 33.58 mm Hg in the UW group, 104.50 ± 72.93 mm Hg in the Celsior group, and 62.08 ± 31.34 mm Hg in PFC and UW solution plus O2 group (two layers). The mean net myeloperoxidase activity OD level was 0.110 ± 0.104 in the LR group, 0.392 ± 0.328 in the UW group, 0.351 ± 0.620 in the Celsior group, and 0.532 ± 0.616 in the two-layer group. The mean wet-to-dry ratio was 7.47 ± 1.60 in the LR group, 6.56 ± 0.62 in the UW group, 7.54 ± 2.19 in the Celsior group, and 5.32 ± 2.20 in the two-layer group. The differences between groups in these parameters were not significant. Upon histologic examination, more inflammatory cell aggregates were seen in the two-layer group, less in the LR and the Celsior groups. The function of the lung graft after 6 hours of storage was not better using this two-layer method for preservation than traditional preservation methods in rat lung transplantation. Histologic examination revealed more inflammatory cell aggregates in the lung graft preserved using a two-layer method.
UR - http://www.scopus.com/inward/record.url?scp=37049006252&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2007.06.085
DO - 10.1016/j.transproceed.2007.06.085
M3 - 文章
C2 - 18089313
AN - SCOPUS:37049006252
SN - 0041-1345
VL - 39
SP - 3019
EP - 3023
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 10
ER -