Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers

Feng Jung Chen; Chia Han Chan; Kuo Liang Liu; Ying Jung Huang; Hwei Ling Peng; Hwan You Chang; Tri Rung Yew; Ken Y. Hsu; Long Hsu

doi:10.1117/12.733097

Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers

Feng Jung Chen^*, Chia Han Chan, Kuo Liang Liu, Ying Jung Huang, Hwei Ling Peng, Hwan You Chang, Tri Rung Yew, Ken Y. Hsu, Long Hsu

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Pili are bacterial appendages that play many important roles in bacterial behaviors, physiology and interaction with hosts. Via pili, bacteria are able to adhere to, migrate onto, and colonize on host cells, mechanically. Different from the most studied type 1 and P type pili, which are rigid and thick with an average of 6-7 nm in diameter, type 3 pili are relatively tiny (3-5 nm in diameter) and flexible, and their biophysical properties remains unclear. By using optical tweezers, we found that the elongation processes of type 3 pili are divided into three phases: (1) elastic elongation, (2) uncoiling elongation, and (3) intrinsic elongation, separately. Besides, the uncoiling force of the recombinant pili displayed on the surface of E. coli [pmrkABCD_v1F] is measured 20 pN in average stronger than that of E. coli [pmrkABCD _v1]. This suggests that pilin MrkF is involved in determining the mechanical properties of the type 3 pili.

Original language	English
Title of host publication	Optical Trapping and Optical Micromanipulation IV
DOIs	https://doi.org/10.1117/12.733097
State	Published - 2007
Externally published	Yes
Event	Optical Trapping and Optical Micromanipulation IV - San Diego, CA, United States Duration: 26 08 2007 → 29 08 2007

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6644
ISSN (Print)	0277-786X

Conference

Conference	Optical Trapping and Optical Micromanipulation IV
Country/Territory	United States
City	San Diego, CA
Period	26/08/07 → 29/08/07

Keywords

Mechanical properties
Optical tweezers
Type 3 pili
Uncoiling force

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10.1117/12.733097

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Chen, F. J., Chan, C. H., Liu, K. L., Huang, Y. J., Peng, H. L., Chang, H. Y., Yew, T. R., Hsu, K. Y., & Hsu, L. (2007). Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers. In Optical Trapping and Optical Micromanipulation IV Article 66441W (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6644). https://doi.org/10.1117/12.733097

@inproceedings{ad038ce9163d48ff9ebd38318b108320,

title = "Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers",

abstract = "Pili are bacterial appendages that play many important roles in bacterial behaviors, physiology and interaction with hosts. Via pili, bacteria are able to adhere to, migrate onto, and colonize on host cells, mechanically. Different from the most studied type 1 and P type pili, which are rigid and thick with an average of 6-7 nm in diameter, type 3 pili are relatively tiny (3-5 nm in diameter) and flexible, and their biophysical properties remains unclear. By using optical tweezers, we found that the elongation processes of type 3 pili are divided into three phases: (1) elastic elongation, (2) uncoiling elongation, and (3) intrinsic elongation, separately. Besides, the uncoiling force of the recombinant pili displayed on the surface of E. coli [pmrkABCDv1F] is measured 20 pN in average stronger than that of E. coli [pmrkABCD v1]. This suggests that pilin MrkF is involved in determining the mechanical properties of the type 3 pili.",

keywords = "Mechanical properties, Optical tweezers, Type 3 pili, Uncoiling force",

author = "Chen, {Feng Jung} and Chan, {Chia Han} and Liu, {Kuo Liang} and Huang, {Ying Jung} and Peng, {Hwei Ling} and Chang, {Hwan You} and Yew, {Tri Rung} and Hsu, {Ken Y.} and Long Hsu",

year = "2007",

doi = "10.1117/12.733097",

language = "英语",

isbn = "9780819467928",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Optical Trapping and Optical Micromanipulation IV",

note = "Optical Trapping and Optical Micromanipulation IV ; Conference date: 26-08-2007 Through 29-08-2007",

}

Chen, FJ, Chan, CH, Liu, KL, Huang, YJ, Peng, HL, Chang, HY, Yew, TR, Hsu, KY & Hsu, L 2007, Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers. in Optical Trapping and Optical Micromanipulation IV., 66441W, Proceedings of SPIE - The International Society for Optical Engineering, vol. 6644, Optical Trapping and Optical Micromanipulation IV, San Diego, CA, United States, 26/08/07. https://doi.org/10.1117/12.733097

Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers. / Chen, Feng Jung; Chan, Chia Han; Liu, Kuo Liang et al.
Optical Trapping and Optical Micromanipulation IV. 2007. 66441W (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6644).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers

AU - Chen, Feng Jung

AU - Chan, Chia Han

AU - Liu, Kuo Liang

AU - Huang, Ying Jung

AU - Peng, Hwei Ling

AU - Chang, Hwan You

AU - Yew, Tri Rung

AU - Hsu, Ken Y.

AU - Hsu, Long

PY - 2007

Y1 - 2007

N2 - Pili are bacterial appendages that play many important roles in bacterial behaviors, physiology and interaction with hosts. Via pili, bacteria are able to adhere to, migrate onto, and colonize on host cells, mechanically. Different from the most studied type 1 and P type pili, which are rigid and thick with an average of 6-7 nm in diameter, type 3 pili are relatively tiny (3-5 nm in diameter) and flexible, and their biophysical properties remains unclear. By using optical tweezers, we found that the elongation processes of type 3 pili are divided into three phases: (1) elastic elongation, (2) uncoiling elongation, and (3) intrinsic elongation, separately. Besides, the uncoiling force of the recombinant pili displayed on the surface of E. coli [pmrkABCDv1F] is measured 20 pN in average stronger than that of E. coli [pmrkABCD v1]. This suggests that pilin MrkF is involved in determining the mechanical properties of the type 3 pili.

AB - Pili are bacterial appendages that play many important roles in bacterial behaviors, physiology and interaction with hosts. Via pili, bacteria are able to adhere to, migrate onto, and colonize on host cells, mechanically. Different from the most studied type 1 and P type pili, which are rigid and thick with an average of 6-7 nm in diameter, type 3 pili are relatively tiny (3-5 nm in diameter) and flexible, and their biophysical properties remains unclear. By using optical tweezers, we found that the elongation processes of type 3 pili are divided into three phases: (1) elastic elongation, (2) uncoiling elongation, and (3) intrinsic elongation, separately. Besides, the uncoiling force of the recombinant pili displayed on the surface of E. coli [pmrkABCDv1F] is measured 20 pN in average stronger than that of E. coli [pmrkABCD v1]. This suggests that pilin MrkF is involved in determining the mechanical properties of the type 3 pili.

KW - Mechanical properties

KW - Optical tweezers

KW - Type 3 pili

KW - Uncoiling force

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SN - 9780819467928

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Optical Trapping and Optical Micromanipulation IV

T2 - Optical Trapping and Optical Micromanipulation IV

Y2 - 26 August 2007 through 29 August 2007

ER -

Uncoiling mechanism of klebsiella pneumoniae type 3 pili measured by using optical tweezers

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