Urinary fluorescence in situ hybridization assay for detecting urothelial carcinoma in Taiwanese patients

Kun Lung Chuang, Hun Chang Chuang, Kwai Fong Ng, Ying Hsun Chang, Chun Te Wu, Cheng Keng Chuang, Shuen Kuei Liao, See Tong Pang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

6 Scopus citations

Abstract

Objectives To investigate the cytogenetic marker detected by fluorescence in situ hybridization (FISH; UroVysionTM, Vysis, Inc., Abbott Laboratories, Des Plaines, IL, USA) in the diagnosis of bladder cancer and upper tract urothelial carcinoma (UC) in Taiwanese patients, as FISH has been used in Western countries for detecting UC, but there are limited Results in Asian populations. Patients and Methods We analysed polyploidy of chromosome 3, 7, 17 and aneuploidy of chromosome 21, using uroepithelial cells collected at the first void or by instrumental extraction of urine, for bladder cancer, and shedding cells from the upper tract flushed by normal saline via ureteric catheterization or ureterorenoscopy. The criteria for positive tumour cells included three or more positive staining in two or more chromosomes showing polyploidy or <50% staining of the chromosome 9p21. Results In all, 32 patients with bladder UC and 12 with upper tract UC were assessed. The overall sensitivity for bladder cancer by UroVysion was 96.8%. The sensitivity of the cytology test was 36% for UC of the bladder. The sensitivity for UroVysion in upper tract UC was 12/12 but the specificity was three of nine. Conclusions This preliminary report shows that UroVysion was a sensitive screening method for UC of the bladder and upper urinary tract.

Original languageEnglish
Pages (from-to)1413-1416
Number of pages4
JournalBJU International
Volume105
Issue number10
DOIs
StatePublished - 05 2010
Externally publishedYes

Keywords

  • Bladder cancer
  • Cytogenetic
  • Fluorescence in situ hybridization
  • Urothelial carcinoma

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