Urinary nucleosides as biomarkers of breast, colon, lung, and gastric cancer in Taiwanese e81701

Wei Yi Hsu, Chao Jung Chen, Yu Chuen Huang, Fuu Jen Tsai, Long Bin Jeng, Chien Chen Lai

Research output: Contribution to journalJournal Article peer-review

48 Scopus citations


Urinary nucleosides are associated with many types of cancer. In this study, six targeted urinary nucleosides, namely adenosine, cytidine, 3-methylcytidine, 1-methyladenosine, inosine, and 2-deoxyguanosine, were chosen to evaluate their role as biomarkers of four different types of cancer: lung cancer, gastric cancer, colon cancer, and breast cancer. Urine samples were purified using solid-phase extraction (SPE) and then analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The Mann-Whitney U test and Principal Component Analysis (PCA) were used to compare differences in urinary nucleosides between patients with one of four types of cancer and healthy controls. The diagnostic sensitivity of single nucleosides for different types of cancer ranged from 14% to 69%. In contrast, the diagnostic sensitivity of a set of six nucleosides ranged from 37% to 69%. The false-positive identification rate associated with the set of six nucleosides in urine was less than 2% compared with that of less than 5% for a single nucleoside. Furthermore, combining the set of six urinary nucleosides with carcinoembryonic antigen improved the diagnostic sensitivity for colon cancer. In summary, the study show that a set of six targeted nucleosides is a good diagnostic marker for breast and colon cancers but not for lung and gastric cancers.

Original languageEnglish
Article numbere81701
JournalPLoS ONE
Issue number12
StatePublished - 19 12 2013
Externally publishedYes


Dive into the research topics of 'Urinary nucleosides as biomarkers of breast, colon, lung, and gastric cancer in Taiwanese e81701'. Together they form a unique fingerprint.

Cite this