Use of surface molecules and receptors for studying macrophages and mononuclear phagocytes

Iain Fraser, Anthony Doyle, Derralynn Hughes, Siamon Gordon*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

13 Scopus citations


Members of the mononuclear phagocyte system use their extensive repertoire of cell surface receptors to interact with their external environment. A number of different assays are available for the study of these molecules and their many functions. In this paper we describe how monoclonal antibodies may be generated against macrophage molecules, and discuss how screening stategies aimed at producing functionally active reagents may be devised. Using the macrophage mannose and scavenger receptors as examples, we describe assays for determining macrophage adhesion to culture plastic surface, endocytosis of soluble ligands and phagocytosis of particles in vitro. These assays may be used in the production of inhibitory monoclonal antibodies, as well as in studies of the regulation of macrophage phenotype and activity.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalJournal of Immunological Methods
Issue number1-2
StatePublished - 14 09 1994
Externally publishedYes


  • Adhesion
  • Endocytosis
  • Macrophage
  • Macrophage mannose receptor
  • Macrophage scavenger receptor
  • Monoclonal antibody
  • Phagocytosis
  • Rosetting


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