Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic adults

Gwo Hwa Wan; Dah Chin Yan; Hui Yun Tseng; Jian Tao Lee; Yu Wen Lin

doi:10.1016/j.jfma.2013.12.009

Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B₄ level in nonatopic adults

Gwo Hwa Wan, Dah Chin Yan, Hui Yun Tseng, Jian Tao Lee, Yu Wen Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

1 Scopus citations

Abstract

This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B₄ (LTB₄) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB₄ concentration in the concentrated pooled EBC samples was 1.19ng/μL, and the average LTB₄ concentration of each EBC sample was 15.38ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB₄ in human EBCs without any complicated sample pretreatment processes.

Original language	English
Pages (from-to)	566-568
Number of pages	3
Journal	Journal of the Formosan Medical Association
Volume	113
Issue number	8
DOIs	https://doi.org/10.1016/j.jfma.2013.12.009
State	Published - 08 2014

Keywords

Exhaled breath condensate
High-performance liquid chromatography
Leukotrienes

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10.1016/j.jfma.2013.12.009

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title = "Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic adults",

abstract = "This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19ng/μL, and the average LTB4 concentration of each EBC sample was 15.38ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.",

keywords = "Exhaled breath condensate, High-performance liquid chromatography, Leukotrienes",

author = "Wan, {Gwo Hwa} and Yan, {Dah Chin} and Tseng, {Hui Yun} and Lee, {Jian Tao} and Lin, {Yu Wen}",

year = "2014",

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AU - Wan, Gwo Hwa

AU - Yan, Dah Chin

AU - Tseng, Hui Yun

AU - Lee, Jian Tao

AU - Lin, Yu Wen

PY - 2014/8

Y1 - 2014/8

N2 - This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19ng/μL, and the average LTB4 concentration of each EBC sample was 15.38ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.

AB - This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19ng/μL, and the average LTB4 concentration of each EBC sample was 15.38ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.

KW - Exhaled breath condensate

KW - High-performance liquid chromatography

KW - Leukotrienes

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AN - SCOPUS:84904391329

SN - 0929-6646

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JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

IS - 8

ER -

Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B₄ level in nonatopic adults

Abstract

Keywords

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